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1.14.18.1: tyrosinase

This is an abbreviated version!
For detailed information about tyrosinase, go to the full flat file.

Word Map on EC 1.14.18.1

Reaction

2 L-dopa +

O2
= 2 dopaquinone + 2 H2O

Synonyms

AbPPO1, AbPPO4, AbTYR, aurone synthase, catalase-phenol oxidase, catechol oxidase, catecholase, CATPO, chlorogenic acid oxidase, chlorogenic oxidase, cresolase, cresolase/monophenolase, CsPPO, CZA14Tyr, deoxy-tyrosinase, dihydroxy-L-phenylalanine:oxygen oxidoreductase, Diphenol oxidase, diphenolase, dopa oxidase, EC 1.10.3.1, EC 1.14.17.2, Hc-derived phenoloxidase, Hc-phenoloxidase, HcPO, HdPO, hemocyanin-derived phenoloxidase, jrPPO1, jrTYR, L-DOPA monophenolase, L-DOPA oxidase, L-DOPA:oxygen oxidoreductase, L-tyrosine hydroxylase, MdPPO1, melC2, MelC2 tyrosinase, met-tyrosinase, monophenol dihydroxyphenylalanine:oxygen oxidoreductase, monophenol monooxidase, monophenol monooxygenase, monophenol monoxygenase, monophenol oxidase, monophenol oxygen oxidoreductase, monophenol, 3,4-dihydroxy L-phenylalanine (L-DOPA):oxygen oxidoreductase, monophenol, dihydroxy-L-phenylalanine oxygen oxidoreductase, monophenol, dihydroxy-L-phenylalanine:oxygen oxidoreductase, monophenol, dihydroxyphenylalanine:oxygen oxidoreductase, monophenol, L-Dopa: oxidoreductase, monophenol, L-DOPA: oxygen oxidoreductase, monophenol, o-diphenol: oxygen oxidoreductase, monophenol, o-diphenol:O2 oxidoreductase, monophenol, o-diphenol:oxygen oxido-reductase, monophenol, o-diphenol:oxygen oxidoreductase, monophenol, polyphenol oxidase, monophenol: dioxygen oxidoreductases, hydroxylating, monophenolase, monphenol mono-oxygenase, More, mTyr, murine tyrosinase, mushroom tyrosinase, mushroom tyrosine, N-acetyl-6-hydroxytryptophan oxidase, o-diphenol oxidase, o-diphenol oxidoreductase, o-diphenol oxygen oxidoreductase, o-diphenol: O2 oxidoreductase, o-diphenol: oxidoreductase, o-diphenol:O2 oxidoreductase, o-diphenol:oxygen oxidoreductase, o-diphenolase, OCA1, Orf13, oxygen oxidoreductase, phenol oxidase, phenol oxidases, phenolase, phenoloxidase, PO, polyaromatic oxidase, polyphenol oxidase, polyphenol oxidase 3, polyphenol oxidase 4, polyphenol oxidase B, polyphenolase, polyphenoloxidase, PotPPO, PPO, PPO 3, PPO B, PPO1, PPO2, PPO3, pro-PO III, prophenoloxidase III, pyrocatechol oxidase, SPRTyr, ST94, ST94t, tryosinase, tryrosinase, TY, tyr, TYR1, TYR2, tyrA, TyrBm, tyrosinase, tyrosinase 2, tyrosinase 4, tyrosinase diphenolase, tyrosine-dopa oxidase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.18 With another compound as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.18.1 tyrosinase

Storage Stability

Storage Stability on EC 1.14.18.1 - tyrosinase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 0.02% bovine serum albumin
-
-20°C, 0.02% bovine serum albumine, no loss of activity
-
-20°C, 10 mM phosphate buffer, pH 7.2, 500 mM NaCl, 30% glycerol, more than 6 months, no loss of activity
-
-20°C, 50 mM phosphate buffer, pH 7.0, more than 3 months, no loss of activity
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-20°C, acetone powder, long term storage
-
-20°C, crude enzyme, several days
-20°C, several weeks, no loss of activity
-
-40°C, 2 months, 60% loss of activity
-
-40°C, enzyme extract, pH 5.0, over 3 months, no loss of activity
-
0°C, presence of SDS protects against decomposition
-
4°C, 1 mM phosphate buffer, pH 7.0
-
4°C, 15 days, 10% loss of activity
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4°C, 2 weeks, 10% loss of activity
-
4°C, 3 weeks, no loss of activity
-
4°C, 36 mM phosphate, 720 mM KCl, pH 7.0, 15 days, 25% loss of activity, remaining activity stable for 120 days, 50% inactivation by thawing after freezing at -40°C
-
4°C, 40% loss of activity after 30 days
-
4°C, 55% of the maximum activity at this cold-storage temperature
-
4°C, 60% ammonium sulfate, several months, no loss of activity
-
4°C, achieves full activity in two days without addition of trypsin, 2.5-fold activation at -20 C in 2 months
-
4°C, pH 5.0/7.0, 5 days, monophenolase/diphenolase activities of the enzyme are conserved approximately 40% and 60%, respectively
-
4°C, purified recombinant detagged enzyme, after 1 year of storage in 10 mM HEPES, pH 7.5, the enzyme is still active
4°C, saturated ammonium sulfate solution
-
catecholase activity is only detectable on freshly collected fractions and is rapidly lost upon storage, whether at 4 or -20°C.
-
liquid N2 or -70°C, phosphate buffer pH 7.2, 150 mM NaCl, several months, no loss of activity
-
PPO is relatively stable at -15°C for 44 days.
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Shelf life of immobilized tyrosinase system is found to be more than 6 months when stored in phosphate buffer at 4°C. Immobilization on preactivated eggshell membrane followed by enzyme adsorption is a simple and a fast procedure to prepare stable immobilized tyrosinase.
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