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0.102
2,4,5-trihydroxyphenethylamine
-
-
0.37
2-caffeoylisocitric acid
-
apparent value
0.82
2-caffeoylisocitric acid 6-methyl ester
-
apparent value
2.7
2-Methylresorcinol
-
pH 7.0, 25°C
0.094
3,4,5-trihydroxyphenethylamine
-
-
0.305
3,4,6-trihydroxyphenylalanine
-
-
0.8
3,4-dihydroxyhydrocinnamic acid
-
at pH 7.0 and 22°C
0.047
3,4-Dihydroxyphenethylamine
-
-
1.89 - 5.1
3,4-dihydroxyphenyl propionic acid
1.3 - 19
3,4-dihydroxyphenylacetic acid
1.96
3,4-dihydroxyphenylalanine
-
-
0.046
3,4-dihydroxyphenylalanine methyl ester
-
-
0.02
3,4-dihydroxyphenylethanol
-
-
0.23
3,4-dihydroxyphenylglycol
-
-
0.24 - 0.7
3,4-Dihydroxyphenylpropionic acid
0.145
3,4-dihydroxyphenylserine
-
-
1240
3-(3,4-dihydroxyphenyl) propionic acid
-
0.194
3-(3,4-Dihydroxyphenyl)-2-methylalanine
-
-
1.25
3-(3,5-dihydroxyphenyl)-1-propanoic acid
recombinant enzyme, pH 7.2, 37°C
2.89
3-(p-hydroxyphenyl)propionic acid
-
-
11
3-chlorophenol
-
in 100 mM phosphate buffer, pH 6.8, at 25°C
0.78
3-hydroxyanthranilic acid
-
-
10.1
3-Hydroxybenzyl alcohol
-
-
0.2
3-hydroxyphloridzin
-
-
0.71
3-[2-(3,4-dihydroxyphenyl)ethylaminocarbonyl]-3-methyl-1-(4-ethoxycarbonylphenyl)triazene
-
in phosphate buffer (pH 7.4), at 37°C
0.31
3-[2-(4-hydroxyphenyl)ethylaminocarbonyl]-3-methyl-1-(4-cyanophenyl)triazene
-
in phosphate buffer (pH 7.4), at 37°C
1.98
4-Bromophenol
-
pH and temperature not specified in the publication
0.35 - 1.87
4-Chlorophenol
0.117
4-ethylresorcinol
-
pH 7.0, 25°C
0.41 - 2.9
4-Fluorophenol
1.14 - 6.37
4-hydroxybenzaldehyde
6.33
4-hydroxybenzoic acid
-
-
1.1 - 14.77
4-Hydroxybenzyl alcohol
0.44 - 0.48
4-hydroxyphenyl propionic acid
0.18 - 1.91
4-hydroxyphenyl acetic acid
0.44
4-hydroxyphenyl propionic acid
-
-
0.22 - 6.7
4-hydroxyphenylpropionic acid
2.02
4-iodophenol
-
pH and temperature not specified in the publication
2.62
4-methylbenzene-1,2-diol
-
apparent value
0.1 - 2000
4-methylcatechol
0.38
4-Methylphenol
-
pH and temperature not specified in the publication
0.203
4-methylresorcinol
-
pH 7.0, 25°C
0.263 - 2.8
4-tert-butylcatechol
0.016 - 0.45
4-tert-butylphenol
2
4-[(1R)-2-amino-1-hydroxyethyl]benzene-1,2-diol
-
-
1.45
Adrenaline bitartrate
-
-
6.5
alpha-arbutin
-
pH 7.0, 25°C
1.12
alpha-methyl-DL-tyrosine
-
-
2.5
alpha-Methyl-dopa
-
-
3.07
alpha-methyl-L-tyrosine
-
-
0.133
ascorbic acid
-
electron donor ascorbic acid
0.037 - 174.5
caffeic acid
0.49 - 129.5
chlorogenic acid
4.6
Coke gallic acid
-
pH 6.8, 30°C
-
3.22 - 6.67
D-epicatechin
0.033
deoxyarbutin
pH and temperature not specified in the publication
2.86
diphenol
Helix vulgaris
-
the Km value is very close to those of Helix pomatia and Sepia officinalis Hcs, but several times higher compared to those of Rapana and Octopus Hcs
1.2
DL-2-methyltyrosine
-
-
3.03 - 5.26
DL-epicatechin
2
epicatechin gallate
-
-
2.2 - 28
epigallocatechin
2 - 32
epigallocatechin gallate
0.071
gallate
-
pH 8.5, 15°C
7.8
gamma-L-glutaminyl-3,4-dihydroxybenzene
-
-
0.3
gamma-L-Glutaminyl-4-hydroxybenzene
-
-
25.6
guaiacolum
-
pH 6.8, 30°C
1
H2O2
-
at pH 8.0 and 37°C
0.67 - 27.4
Hydrocaffeic acid
0.92
hydroquinone
-
pH 7.0, 25°C
2 - 14
Hydroquinone monomethylether
-
range of estimated values for crude or purified extracts
0.4
Hydroxyhydroquinone
-
pH and temperature not specified in the publication
1.2
L-alpha-methyl tyrosine
-
-
2.5 - 6.8
L-alpha-methyldopa
1.9 - 4.2
L-dopa methyl ester
3.85 - 11.1
L-epicatechin
7.1 - 230
L-isoproterenol
0.38 - 1.66
L-tyrosine methyl ester
0.28 - 0.82
methyl 3-hydroxy-L-tyrosinate
0.13
methylgallate
-
pH 8.5, 15°C
0.014
N-acetyl-3,4-dihydroxyphenethylamine
-
-
0.48
N-acetyldopamine
-
-
0.1
N-beta-alanyldopamine
-
-
8
N-Formyl-L-tyrosine
-
-
0.024
N-methyl-3,4-dihydroxyphenethylamine
-
-
0.00865
oxyresveratrol
-
pH 7.0, 25°C
0.268 - 4
p-coumaric acid
0.64 - 1.4
phloretic acid
0.07 - 189
protocatechuic acid
0.15 - 2.87
Protocatechuic aldehyde
0.46
resorcinol
-
pH 7.0, 25°C
1.2
tert-butylcathechol
-
-
0.54
trans-cinnamic acid
-
pH 3.5, 25°C
additional information
additional information
-
6.1
(+)-catechin
-
at pH 4.0, slight increase in Km above pH 4.0
6.6
(+)-catechin
-
at pH 5.5, slight increase in Km above pH 4.0
1.89
3,4-dihydroxyphenyl propionic acid
-
-
5.1
3,4-dihydroxyphenyl propionic acid
-
-
1.3
3,4-dihydroxyphenylacetic acid
-
-
11
3,4-dihydroxyphenylacetic acid
recombinant enzyme, pH 7.2, 37°C
19
3,4-dihydroxyphenylacetic acid
recombinant enzyme, pH 7.2, 37°C
0.24
3,4-Dihydroxyphenylpropionic acid
pH 6.8, 25°C, ST94t
0.49
3,4-Dihydroxyphenylpropionic acid
pH 6.8, 25°C, ST94
0.7
3,4-Dihydroxyphenylpropionic acid
-
-
0.35
4-Chlorophenol
-
in 100 mM phosphate buffer, pH 6.8, at 25°C
1.87
4-Chlorophenol
-
pH and temperature not specified in the publication
0.17
4-ethoxyphenol
-
-
0.17
4-ethoxyphenol
-
pH 7.0, 25°C
0.17
4-ethoxyphenol
-
pH and temperature not specified in the publication
0.41
4-Fluorophenol
-
pH and temperature not specified in the publication
0.08
4-hydroxyanisole
-
-
0.08
4-hydroxyanisole
-
pH 7.0, 25°C
0.08
4-hydroxyanisole
-
pH and temperature not specified in the publication
1.14
4-hydroxybenzaldehyde
-
pH and temperature not specified in the publication
6.37
4-hydroxybenzaldehyde
-
-
1.1
4-Hydroxybenzyl alcohol
-
-
14.77
4-Hydroxybenzyl alcohol
-
-
0.44
4-hydroxyphenyl propionic acid
-
-
0.48
4-hydroxyphenyl propionic acid
-
-
0.18
4-hydroxyphenyl acetic acid
-
-
1.16
4-hydroxyphenyl acetic acid
-
-
1.91
4-hydroxyphenyl acetic acid
-
-
0.22
4-hydroxyphenylpropionic acid
-
pH 6.8, 10°C, in methanol-glycerol cryosolvent
0.44
4-hydroxyphenylpropionic acid
-
pH 6.8, 25°C, in aqueous Hepes buffer
1
4-hydroxyphenylpropionic acid
-
at pH 7.0 and 22°C
6.7
4-hydroxyphenylpropionic acid
-
pH 6.8, 25°C, in methanol-glycerol cryosolvent
0.08
4-methoxyphenol
-
-
0.08
4-methoxyphenol
-
pH 7.0, 25°C
4 - 5
4-methyl catechol
-
in poly-(10)-oxyethylene oleyl ether-cyclohexane reverse micelles
21
4-methyl catechol
-
polyphenol oxidase shows the best activity toward catechol and 4-methyl catechol
0.1
4-methylcatechol
-
-
0.15
4-methylcatechol
-
pH 6.5, 28°C
0.17
4-methylcatechol
Mushroom
-
-
0.17
4-methylcatechol
Mushroom
-
in H2O
0.2
4-methylcatechol
Mushroom
-
measured in bis(2-ethylhexyl)sodium sulfosuccinate-isooctane reverse micelles
0.2
4-methylcatechol
Mushroom
-
in MOPS buffer, pH 7.0
0.3
4-methylcatechol
-
in aqueous solution
0.5
4-methylcatechol
-
in CHCl3
1.15
4-methylcatechol
-
-
2.36
4-methylcatechol
-
-
2.5
4-methylcatechol
recombinant enzyme, pH 7.2, 37°C
2.8
4-methylcatechol
-
pH 8.0, 20°C
3.14
4-methylcatechol
-
pH 7.0, 25°C
4
4-methylcatechol
Mushroom
-
in ternary solution composed of hexane-isopropanol-water
4.75
4-methylcatechol
-
-
5.13
4-methylcatechol
-
-
5.2
4-methylcatechol
-
at pH 3.5-5.0, strong increase in Km above pH 5.0
10
4-methylcatechol
-
pH 7.0, 50°C
15
4-methylcatechol
Mushroom
-
in ternary solution composed of toluene-isopropanol-water
20.5
4-methylcatechol
-
at pH 6.3, strong increase in Km above pH 5.0
33
4-methylcatechol
-
in 1-butyl-3-methylimidazolium hexafluorophosphate, much higher than those obtained in CHCl3 and aqueous solution
2000
4-methylcatechol
-
-
0.263
4-tert-butylcatechol
-
soluble (PPO-S) peach polyphenol oxidase without SDS
0.3
4-tert-butylcatechol
-
membrane-bound (PPO-B) peach polyphenol oxidase without SDS
0.6
4-tert-butylcatechol
recombinant enzyme, pH 7.2, 37°C
0.65
4-tert-butylcatechol
recombinant enzyme, pH 7.2, 37°C
0.85
4-tert-butylcatechol
-
membrane-bound (PPO-B) peach polyphenol oxidase with 2 mM SDS
1
4-tert-butylcatechol
-
-
1.45
4-tert-butylcatechol
-
-
1.84
4-tert-butylcatechol
-
soluble (PPO-S) peach polyphenol oxidase with 2 mM SDS
2.8
4-tert-butylcatechol
-
-
0.016
4-tert-butylphenol
-
-
0.45
4-tert-butylphenol
-
pH and temperature not specified in the publication
3
beta-arbutin
-
pH 7.0, 25°C
3
beta-arbutin
pH and temperature not specified in the publication
0.037
caffeic acid
-
pH 8.5, 15°C
0.9
caffeic acid
-
in 0.1M sodium phosphate buffer (pH 7.0) at 25°C
1.69
caffeic acid
-
in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C
174.5
caffeic acid
-
at pH 6.0, 20°C
0.479
catechin
pH and temperature not specified in the publication
0.014
catechol
-
isoenzyme A3
0.014
catechol
-
isozyme A3, at pH 6.0 and 30°C
0.018
catechol
-
isoenzyme B3
0.018
catechol
-
isozyme B3, at pH 6.0 and 30°C
0.087
catechol
-
isoenzyme B1
0.087
catechol
-
isozyme B1, at pH 6.0 and 30°C
0.09
catechol
-
isoenzyme A1
0.09
catechol
-
isozyme A1, at pH 6.0 and 30°C
0.091
catechol
-
isoenzyme A2
0.091
catechol
-
isozyme A2, at pH 6.0 and 30°C
0.104
catechol
-
isoenzyme B2
0.104
catechol
-
isozyme B2, at pH 6.0 and 30°C
0.25
catechol
-
in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C
0.28
catechol
-
pH 8.5, 15°C
0.604
catechol
pH 7.0, 20°C
2 - 3
catechol
pH 5.6, 37°C
2 - 3
catechol
-
detected in dormant saffron corms
2.17
catechol
-
calculated from the Lineweaver-Burk graphs for substrates
2.5
catechol
-
in 0.1M sodium phosphate buffer (pH 7.0) at 25°C
3.1
catechol
pH and temperature not specified in the publication
3.8
catechol
-
in 0.2 mM phosphate buffer at pH 6.8 and 30°C
4.5
catechol
recombinant enzyme, pH 7.2, 37°C
4.65
catechol
pH 6.8, 25°C, recombinant enzyme
5
catechol
-
isoenzymes A and B
6.47
catechol
-
pH 6.5, 28°C
8
catechol
-
pH 7.0, 50°C
12.34
catechol
-
polyphenol oxidase shows the best activity toward catechol and 4-methyl catechol
15.14
catechol
-
pH 7.0, 25°C
15.4
catechol
-
at pH 6.0, 20°C
18
catechol
-
pH 6.8, 30°C
32
catechol
recombinant enzyme, pH 7.2, 37°C
34.05
catechol
-
pH 8.5, 42°C
0.49
chlorogenic acid
-
-
0.53
chlorogenic acid
-
pH 6.0, 30°C
0.835
chlorogenic acid
-
PPO of dill has a great affinity towards chlorogenic acid. calculated from the Lineweaver-Burk graphs for substrates
1.19
chlorogenic acid
pH 6.0, 30°C
1.5
chlorogenic acid
-
pH 6.8, 30°C
1.91
chlorogenic acid
pH 6.0, 30°C
3.47
chlorogenic acid
-
apparent value
4.1
chlorogenic acid
-
at pH 4.0, slight increase in Km above pH 5.0
4.9
chlorogenic acid
-
at pH 5.5, slight increase in Km above pH 5.0
5.15
chlorogenic acid
-
-
129.5
chlorogenic acid
-
at pH 7.0, 30°C
3
D-catechin
-
isoenzyme III
5
D-catechin
-
isoenzyme I
0.026
D-Dopa
-
-
1.18
D-Dopa
-
in 50 mM potassium phosphate buffer (pH 7.0)
1.2
D-Dopa
-
wild type enzyme, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C
1.7
D-Dopa
-
in 50 mM potassium phosphate buffer (pH 7.0), with 30% (v/v) DMSO
2.7
D-Dopa
pH 6.8, 25°C, ST94t
3.2
D-Dopa
pH 6.8, 25°C, ST94
3.8
D-Dopa
-
mutant enzyme R209H, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C
3.22
D-epicatechin
-
isoenzyme III
6.67
D-epicatechin
-
isoenzyme I
0.24
D-Tyr
pH 6.8, 25°C, ST94t
0.32
D-Tyr
pH 6.8, 25°C, ST94
1.2
D-tyrosine
random mutant enzyme RV145, at pH 7.0 and 30°C
1.9
D-tyrosine
random mutant enzyme RVC10, at pH 7.0 and 30°C
2.2
D-tyrosine
Vibrio tyrosinaticus
-
isoenzyme A
2.4
D-tyrosine
random mutant enzyme C10-N322S, at pH 7.0 and 30°C
3.5
D-tyrosine
random mutant enzyme C10-T183I, at pH 7.0 and 30°C
5.1
D-tyrosine
random mutant enzyme 145-Y119F, at pH 7.0 and 30°C
6.2
D-tyrosine
wild type enzyme, at pH 7.0 and 30°C
6.3
D-tyrosine
random mutant enzyme C10-T359M, at pH 7.0 and 30°C
6.9
D-tyrosine
random mutant enzyme 145-D317Y, at pH 7.0 and 30°C
7.3
D-tyrosine
random mutant enzyme C10-F185Y, at pH 7.0 and 30°C
9.6
D-tyrosine
random mutant enzyme 145-L330V, at pH 7.0 and 30°C
15
D-tyrosine
random mutant enzyme 145-V153A, at pH 7.0 and 30°C
0.022
DL-dopa
-
-
0.48
DL-dopa
Mushroom
-
-
3.03
DL-epicatechin
-
isoenzyme I
5.26
DL-epicatechin
-
isoenzyme III
0.25
Dopa
Sigma mushroom tyrosinase (SMT, crude powder, lot 105k7026)
0.46
Dopa
Worthington mushroom tyrosinase (WMT, crude powder, lot 33H6588Q)
0.77
Dopa
-
enzyme fraction II
0.11
dopamine
-
-
0.2
dopamine
-
soluble enzyme
0.39
dopamine
pH 6.8, 25°C, ST94t
0.4
dopamine
-
pH 6.8, 10°C, in methanol-glycerol cryosolvent
0.67
dopamine
-
using tyrosinase immobilized on eggshell membrane. Km is 0.67 mM and Vmax 0.08 mM min-1. Increase in Km (compared to soluble enzyme for which Km is 0.20 mM) indicate that the affinity of enzyme for substrate has decreased to some extent after immobilization, which may be due to glutaraldehyde treatment
0.84
dopamine
-
in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C
0.892
dopamine
-
pH 8.5, 42°C
0.93
dopamine
-
pH 6.8, 25°C, in methanol-glycerol cryosolvent
1.3
dopamine
pH 6.8, 25°C, ST94
1.67
dopamine
-
calculated from the Lineweaver-Burk graphs for substrates
2.2
dopamine
-
pH 6.8, 25°C, in aqueous Hepes buffer
3.4
dopamine
-
inhibitor is phenylthiourea
4.2
dopamine
-
inhibitor is mimosine
11
dopamine
-
in 0.1M sodium phosphate buffer (pH 7.0) at 25°C
15.2
dopamine
pH 6.8, 25°C, recombinant enzyme
16
dopamine
recombinant enzyme, pH 7.2, 37°C
50
dopamine
recombinant enzyme, pH 7.2, 37°C
0.1
ellagic acid
-
at pH 7.0
0.12
ellagic acid
-
at pH 4.5
0.314
epicatechin
pH and temperature not specified in the publication
2.2
epigallocatechin
-
-
21
epigallocatechin
pH 5.6, 37°C
28
epigallocatechin
pH 5.6, 37°C
2
epigallocatechin gallate
-
-
6.9
epigallocatechin gallate
pH 5.6, 37°C
32
epigallocatechin gallate
pH 5.6, 37°C
0.63
esculetin
-
(KM as a kinetic constant) of the PPO/O2/ESC system at pH 7
0.9
esculetin
-
(KM as a kinetic constant) of the PPO/O2/ESC system at pH 4.5
0.18
gallic acid
-
-
6.8
gallic acid
-
pH 6.8, 30°C
21.2
gallic acid
-
at pH 7.0, 30°C
0.11
Gly-Gly-L-Tyr
-
in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C
3.1
Gly-Gly-L-Tyr
-
in 0.1M sodium phosphate buffer (pH 7.0) at 25°C
0.18
Gly-L-Tyr-Gly
-
in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C
3.9
Gly-L-Tyr-Gly
-
in 0.1M sodium phosphate buffer (pH 7.0) at 25°C
0.67
Hydrocaffeic acid
-
-
0.91
Hydrocaffeic acid
-
in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C
3
Hydrocaffeic acid
-
in 0.1M sodium phosphate buffer (pH 7.0) at 25°C
27.4
Hydrocaffeic acid
-
-
2.5
L-alpha-methyldopa
-
-
6.8
L-alpha-methyldopa
-
-
0.016
L-Dopa
-
-
0.0161
L-Dopa
-
pH 5.0, 25°C
0.074
L-Dopa
-
isoenzyme B3
0.074
L-Dopa
-
isozyme B3, at pH 6.0 and 30°C
0.085
L-Dopa
-
isoenzyme A3
0.085
L-Dopa
-
isozyme A3, at pH 6.0 and 30°C
0.089
L-Dopa
-
isoenzyme A2
0.089
L-Dopa
-
isozyme A2, at pH 6.0 and 30°C
0.17
L-Dopa
-
in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C
0.172
L-Dopa
-
pH 6.8, 25°C
0.185
L-Dopa
-
isoenzyme B2
0.185
L-Dopa
-
isozyme B2, at pH 6.0 and 30°C
0.34
L-Dopa
recombinant enzyme, pH 7.2, 37°C
0.35
L-Dopa
-
in 50 mM potassium phosphate buffer (pH 7.0)
0.36
L-Dopa
-
recombinant enzyme
0.36
L-Dopa
-
in 0.25 M phosphate buffer (pH 6.8), at 30°C
0.4
L-Dopa
-
wild type enzyme, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C
0.41
L-Dopa
-
soluble enzyme, pH 6.0, 55°C
0.48
L-Dopa
-
human tyrosinase source HEK293-TYR
0.49
L-Dopa
-
human tyrosinase source TXM13 pigment cell
0.5
L-Dopa
-
in B16/C3 tumor cell homogenates
0.51
L-Dopa
-
high-mobility enzyme form
0.62
L-Dopa
-
mutant enzyme R209H, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C
0.64
L-Dopa
endogenous enzyme, in 50 mM Tris-HCl (pH 7.0), at 30°C
0.73
L-Dopa
-
in 50 mM potassium phosphate buffer (pH 7.0), with 30% (v/v) DMSO
0.768
L-Dopa
-
pH 8.5, 42°C
0.77
L-Dopa
Helix vulgaris
-
-
0.8
L-Dopa
-
pH 6.8, 37°C
0.8
L-Dopa
-
pH 6.8, 25°C, in aqueous Hepes buffer
0.8
L-Dopa
wild type enzyme, in 50 mM Tris HCl buffer pH 7.5, 0.01 mM CuSO4, at 28°C
0.933
L-Dopa
-
pH 7.0, 35°C
1
L-Dopa
mutant enzyme V218G, in 50 mM Tris HCl buffer pH 7.5, 0.01 mM CuSO4, at 28°C
1.1
L-Dopa
mutant enzyme V218F, in 50 mM Tris HCl buffer pH 7.5, 0.01 mM CuSO4, at 28°C
1.16
L-Dopa
recombinant enzyme, in 50 mM Tris-HCl (pH 7.0), at 30°C
1.16
L-Dopa
-
cross-linked enzyme aggregate, pH 6.0, 30°C
1.2
L-Dopa
-
isoenzyme B1
1.2
L-Dopa
-
isozyme B1, at pH 6.0 and 30°C
1.347
L-Dopa
-
pH 7.4, 30°C
1.44
L-Dopa
-
in 50 mM phosphate buffer, pH 6.5, at 37°C
1.5
L-Dopa
recombinant enzyme, pH 7.2, 37°C
1.5
L-Dopa
-
in 15 mM Tris-HCl at pH 7.8, with 5 mM CaCl2 and 5 mM MgCl2
1.9
L-Dopa
-
low-mobility enzyme form
1.9
L-Dopa
-
preparation shows substrate inhibition kinetics with L-dopa (3,4-dihydroxy-L-phenylalanine)
2.1
L-Dopa
-
pH 6.8, 10°C, in methanol-glycerol cryosolvent
2.2
L-Dopa
-
isoenzyme A1
2.2
L-Dopa
-
isozyme A1, at pH 6.0 and 30°C
2.26
L-Dopa
-
pH 8.5, 15°C
2.9
L-Dopa
-
in 20 mM Tris-HCl buffer (pH 7.1)
3.7
L-Dopa
-
pH 6.8, 25°C, in methanol-glycerol cryosolvent
4.2
L-Dopa
-
detected in dormant saffron corms
6.5
L-Dopa
pH 6.8, 25°C, ST94t
6.8
L-Dopa
pH 6.8, 25°C, ST94
7.5
L-Dopa
-
in 0.1M sodium phosphate buffer (pH 7.0) at 25°C
8.1
L-Dopa
-
His6-tagged TYRC
21.4
L-Dopa
-
pH 3.5, 25°C
26.1
L-Dopa
pH 6.8, 25°C, recombinant enzyme
36
L-Dopa
Vibrio tyrosinaticus
-
isoenzyme A
67
L-Dopa
Vibrio tyrosinaticus
-
isoenzyme B
1.9
L-dopa methyl ester
recombinant enzyme, pH 7.2, 37°C
4.2
L-dopa methyl ester
recombinant enzyme, pH 7.2, 37°C
3.85
L-epicatechin
-
isoenzyme I
11.1
L-epicatechin
-
isoenzyme III
7.1
L-isoproterenol
-
-
58
L-isoproterenol
recombinant enzyme, pH 7.2, 37°C
230
L-isoproterenol
recombinant enzyme, pH 7.2, 37°C
0.21
L-Tyr
-
-
0.35
L-Tyr
pH 6.8, 25°C, ST94t
0.563
L-Tyr
-
pH 8.5, 42°C
0.57
L-Tyr
pH 6.8, 25°C, ST94
0.21
L-Tyr-Gly-Gly
-
in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C
6
L-Tyr-Gly-Gly
-
in 0.1M sodium phosphate buffer (pH 7.0) at 25°C
0.014
L-tyrosine
-
isoenzyme A2
0.014
L-tyrosine
-
isoenzyme A3
0.014
L-tyrosine
-
isoenzyme B1
0.014
L-tyrosine
-
isoenzyme B2
0.014
L-tyrosine
-
isoenzyme B3
0.014
L-tyrosine
-
isozyme A2, A3, B1, B2, or B3, at pH 6,0 and 30°C
0.015
L-tyrosine
-
isoenzyme A1
0.015
L-tyrosine
-
isozyme A1, at pH 6.0 and 30°C
0.038
L-tyrosine
-
wild type enzyme, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C
0.05
L-tyrosine
wild type enzyme, in 50 mM Tris HCl buffer pH 7.5, 0.01 mM CuSO4, at 28°C
0.075
L-tyrosine
-
in 50 mM potassium phosphate buffer (pH 7.0)
0.12
L-tyrosine
-
high-mobility enzyme form
0.17
L-tyrosine
-
recombinant enzyme
0.18
L-tyrosine
-
in 0.25 M phosphate buffer (pH 6.8), at 30°C
0.2
L-tyrosine
-
in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C
0.21
L-tyrosine
-
pH 7.0, 25°C
0.22
L-tyrosine
recombinant enzyme, pH 7.2, 37°C
0.23
L-tyrosine
-
low-mobility enzyme form
0.347
L-tyrosine
-
in 50 mM phosphate buffer, pH 6.5, at 37°C
0.45
L-tyrosine
-
at pH 8.0 and 37°C
0.5
L-tyrosine
mutant enzyme V218G, in 50 mM Tris HCl buffer pH 7.5, 0.01 mM CuSO4, at 28°C
0.6
L-tyrosine
random mutant enzyme RV145, at pH 7.0 and 30°C
0.79
L-tyrosine
-
pH 4.5, 25°C
0.9
L-tyrosine
random mutant enzyme RVC10, at pH 7.0 and 30°C
1
L-tyrosine
random mutant enzyme C10-N322S, at pH 7.0 and 30°C
1.3
L-tyrosine
-
mutant enzyme R209H, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C
1.4
L-tyrosine
-
Vmax (DELTAOD415 nm/min) is 0.015
1.4
L-tyrosine
mutant enzyme V218F, in 50 mM Tris HCl buffer pH 7.5, 0.01 mM CuSO4, at 28°C
2.1
L-tyrosine
wild type enzyme, at pH 7.0 and 30°C
2.4
L-tyrosine
-
in the presence of kuraridinol
2.5
L-tyrosine
-
In the presence of inhibitor diluted to 1/4 of its original concentration, the tyrosinase activity had the same Vmax value of 0.015
2.9
L-tyrosine
-
in the presence of kurarinol
3.1
L-tyrosine
Vibrio tyrosinaticus
-
isoenzyme A and B
3.14
L-tyrosine
pH 6.8, 25°C, recombinant enzyme
4.1
L-tyrosine
-
In the presence of inhibitor diluted to 1/8 of its original concentration, the tyrosinase activity had the same Vmax value of 0.015
5.1
L-tyrosine
recombinant enzyme, pH 7.2, 37°C
7.33
L-tyrosine
-
in 20 mM Tris-HCl buffer (pH 7.1)
77
L-tyrosine
-
at pH 5.8, 30°C
90
L-tyrosine
pH 5.6, 37°C
145
L-tyrosine
pH 5.6, 37°C
0.38
L-tyrosine methyl ester
-
-
1.66
L-tyrosine methyl ester
-
-
0.28
methyl 3-hydroxy-L-tyrosinate
-
pH 6.8, 10°C, in methanol-glycerol cryosolvent
0.38
methyl 3-hydroxy-L-tyrosinate
-
pH 6.8, 25°C, in aqueous Hepes buffer
0.82
methyl 3-hydroxy-L-tyrosinate
-
pH 6.8, 25°C, in methanol-glycerol cryosolvent
23.81
o-Diphenol
-
Rapana venosa RvH2 (subunits)
28.57
o-Diphenol
-
Rapana venosa native (didecamers)
0.015
O2
-
at pH 4.5
0.04
O2
-
(KM as a kinetic constant) of the PPO/O2/ESC system at pH 7
0.041
O2
-
(KM as a kinetic constant) of the PPO/O2/ESC system at pH 4.5
0.067
O2
-
electron donor ascorbic acid
1.5
O2
-
substrate gamma-L-glutaminyl-4-hydroxybenzene
2.1
O2
-
substrate L-tyrosine
38.2
O2
-
substrate L-dopa
100.2
O2
-
substrate gamma-L-glutaminyl-3,4-dihydroxybenzene
393
O2
-
using D-ascorbic acid as cosubstrate
421
O2
-
using L-ascorbic acid as cosubstrate
0.268
p-coumaric acid
-
electron donor ascorbic acid
1.5
p-coumaric acid
-
in 0.1M sodium phosphate buffer (pH 7.0) at 25°C
0.001
p-Cresol
-
isoenzyme A3
0.001
p-Cresol
-
isoenzyme B1
0.001
p-Cresol
-
isoenzyme B2
0.001
p-Cresol
-
isoenzyme B3
0.001
p-Cresol
-
isozyme A3, B1, B2, or B3, at pH 6,0 and 30°C
0.002
p-Cresol
-
isoenzyme A1
0.002
p-Cresol
-
isozyme A1, at pH 6.0 and 30°C
0.003
p-Cresol
-
isoenzyme A2
0.003
p-Cresol
-
isozyme A2, at pH 6.0 and 30°C
2.2
p-Cresol
-
pH 7.0, 25°C
130
p-Cresol
-
detected in dormant saffron corms
0.06
p-tyrosol
-
in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C
0.8
p-tyrosol
-
in 0.1M sodium phosphate buffer (pH 7.0) at 25°C
0.33
phenol
-
in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C
0.6 - 1
phenol
-
pH and temperature not specified in the publication
0.7
phenol
-
pH 7.0, 25°C
3.8
phenol
-
in 0.1M sodium phosphate buffer (pH 7.0) at 25°C
12.5
phenol
pH 6.8, 25°C, recombinant enzyme
0.64
phloretic acid
-
in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C
1.4
phloretic acid
-
in 0.1M sodium phosphate buffer (pH 7.0) at 25°C
0.07
protocatechuic acid
-
-
0.37
protocatechuic acid
-
-
189
protocatechuic acid
-
-
0.15
Protocatechuic aldehyde
-
-
0.59
Protocatechuic aldehyde
-
-
2.87
Protocatechuic aldehyde
-
-
0.4
pyrocatechol
-
Lineweaver-Burk plot of pyrocatechol oxidation by the mushroom PPO
7.3
pyrocatechol
pH 6.8, 25°C, ST94t
9.3
pyrocatechol
pH 6.8, 25°C, ST94
0.11
pyrogallate
-
pH 8.5, 15°C
5.8
pyrogallate
pH 5.6, 37°C
8
pyrogallate
pH 5.6, 37°C
0.3
pyrogallol
-
detected in dormant saffron corms
1.4
pyrogallol
-
Lineweaver-Burk plot of pyrogallol oxidation by the mushroom PPO
2.77
pyrogallol
-
pH 7.0, 25°C
20.11
pyrogallol
-
pH 6.5, 28°C
57
pyrogallol
-
at pH 7.0, 30°C
0.0077
tyramine
-
pH 5.0, 25°C
0.12
tyramine
pH 6.8, 25°C, ST94t
0.32
tyramine
pH 6.8, 25°C, ST94
0.51
tyramine
-
pH 6.8, 25°C, in aqueous Hepes buffer
0.66
tyramine
-
pH 6.8, 10°C, in methanol-glycerol cryosolvent
0.75
tyramine
-
in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C
1.6
tyramine
-
inhibitor is mimosine
1.6
tyramine
-
inhibitor is phenylthiourea
2.1
tyramine
-
pH 6.8, 25°C, in methanol-glycerol cryosolvent
4.5
tyramine
-
in 0.1M sodium phosphate buffer (pH 7.0) at 25°C
9.53
tyramine
pH 6.8, 25°C, recombinant enzyme
additional information
additional information
-
4.3% O2
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
detailed kinetics, overview
-
additional information
additional information
-
detailed kinetics, overview
-
additional information
additional information
-
detailed kinetics, overview
-
additional information
additional information
-
detailed kinetics, overview
-
additional information
additional information
-
kinetic mechanism, general kinetic model
-
additional information
additional information
-
kinetics, the activation free energy is dominated by the enthalpic term, the value of which lies in the relatively narrow range of 61 kJmol-1 irrespective of substrate or reaction type as monophenolase, EC 1.14.18.1, or diphenolase
-
additional information
additional information
-
no Michaelis-Menten kinetics
-
additional information
additional information
-
steady-state rate in monophenolase activity
-
additional information
additional information
-
kinetic parameters for free and immobilized enzymes, determined according to the Michaelis-Menten equation, show a lower Km value for PPO-MAC400 than for the free enzyme, indicating higher affinity towards substrate, while PPO-MAC200 exhibits a higher Km value
-
additional information
additional information
MichaelisMenten kinetics
-
additional information
additional information
-
Michaelis-Menten steady-state kinetics
-
additional information
additional information
enzyme kinetic analysis at different pH values, overview
-
additional information
additional information
-
enzyme kinetics
-
additional information
additional information
-
kinetics measurement and simulation of in vitro kinetics of the tyrosinase reaction
-
additional information
additional information
-
Michaeli-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics, kinetic study of tyrosinase by pressure-mediated (electrophoretically-mediated) microanalysis with substrate L-dopa, method development and validation, overview
-
additional information
additional information
-
possible kinetic mechanism for explaining the action of tyrosinase on hydroxyhydroquinone, detailed overview
-