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1.14.18.1: tyrosinase

This is an abbreviated version!
For detailed information about tyrosinase, go to the full flat file.

Word Map on EC 1.14.18.1

Reaction

2 L-dopa +

O2
= 2 dopaquinone + 2 H2O

Synonyms

AbPPO1, AbPPO4, AbTYR, aurone synthase, catalase-phenol oxidase, catechol oxidase, catecholase, CATPO, chlorogenic acid oxidase, chlorogenic oxidase, cresolase, cresolase/monophenolase, CsPPO, CZA14Tyr, deoxy-tyrosinase, dihydroxy-L-phenylalanine:oxygen oxidoreductase, Diphenol oxidase, diphenolase, dopa oxidase, EC 1.10.3.1, EC 1.14.17.2, Hc-derived phenoloxidase, Hc-phenoloxidase, HcPO, HdPO, hemocyanin-derived phenoloxidase, jrPPO1, jrTYR, L-DOPA monophenolase, L-DOPA oxidase, L-DOPA:oxygen oxidoreductase, L-tyrosine hydroxylase, MdPPO1, melC2, MelC2 tyrosinase, met-tyrosinase, monophenol dihydroxyphenylalanine:oxygen oxidoreductase, monophenol monooxidase, monophenol monooxygenase, monophenol monoxygenase, monophenol oxidase, monophenol oxygen oxidoreductase, monophenol, 3,4-dihydroxy L-phenylalanine (L-DOPA):oxygen oxidoreductase, monophenol, dihydroxy-L-phenylalanine oxygen oxidoreductase, monophenol, dihydroxy-L-phenylalanine:oxygen oxidoreductase, monophenol, dihydroxyphenylalanine:oxygen oxidoreductase, monophenol, L-Dopa: oxidoreductase, monophenol, L-DOPA: oxygen oxidoreductase, monophenol, o-diphenol: oxygen oxidoreductase, monophenol, o-diphenol:O2 oxidoreductase, monophenol, o-diphenol:oxygen oxido-reductase, monophenol, o-diphenol:oxygen oxidoreductase, monophenol, polyphenol oxidase, monophenol: dioxygen oxidoreductases, hydroxylating, monophenolase, monphenol mono-oxygenase, More, mTyr, murine tyrosinase, mushroom tyrosinase, mushroom tyrosine, N-acetyl-6-hydroxytryptophan oxidase, o-diphenol oxidase, o-diphenol oxidoreductase, o-diphenol oxygen oxidoreductase, o-diphenol: O2 oxidoreductase, o-diphenol: oxidoreductase, o-diphenol:O2 oxidoreductase, o-diphenol:oxygen oxidoreductase, o-diphenolase, OCA1, Orf13, oxygen oxidoreductase, phenol oxidase, phenol oxidases, phenolase, phenoloxidase, PO, polyaromatic oxidase, polyphenol oxidase, polyphenol oxidase 3, polyphenol oxidase 4, polyphenol oxidase B, polyphenolase, polyphenoloxidase, PotPPO, PPO, PPO 3, PPO B, PPO1, PPO2, PPO3, pro-PO III, prophenoloxidase III, pyrocatechol oxidase, SPRTyr, ST94, ST94t, tryosinase, tryrosinase, TY, tyr, TYR1, TYR2, tyrA, TyrBm, tyrosinase, tyrosinase 2, tyrosinase 4, tyrosinase diphenolase, tyrosine-dopa oxidase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.18 With another compound as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.18.1 tyrosinase

Cloned

Cloned on EC 1.14.18.1 - tyrosinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Agaricus bisporus possesses genes coding for six different tyrosinases (AbPPO1-AbPPO6), full-length gene AbPPO4 (1-565) DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally GST-tagged tyrosinase 4 from gene AbPPO4 containing 23 mutations in Escherichia coli as latent enzyme
cloning of cDNA
DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Escherichia coli strain DH5alpha
DNA and amino acid sequence determination and analysis, sequence comparisons
Escherichia coli CC118gammapir with plasmid mini-Tn5 derivative pUT-Km1 (transposon donor to introduce mutagenisis), Escherichia coli HB101 with plasmid pRK600 (helper), and Escherichia coli TOP 10 as general cloning host for triparental mating experiments with Pseudomonas putida as recipient
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expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in HEK-293T cells
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expressed in Pichia pastoris
expression in Escherichia coli
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expression of wild-type, N86Q, N111Q, N337Q, N371Q and several double and triple mutant enzymes derived from this single mutants in CHO cells
-
gene AS1, DNA and amino acid sequence determination and analyis
gene jrPPO1, DNA and amino acid sequence determination and analysis, sequence comparisons
gene MdPPO1, cloned from apple leaves, recombinant expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain BL21
gene PPO, DNA and amino acid sequence determination and analyis
gene TYR, DNA and amino acid sequence determinatin and analysis, sequence comparisons, recombinant expression of His-tagged intra-melanosomal domain wild-type and mutant variants OCA1A and OCA1B in Trichoplusia ni insect cells using the baculovirus transfection method
gene tyr2, DNA and amino acid sequence determination and analysis, overexpression in the native host under the strong cbh1 promoter, secretion of the recombinant enzyme into the culture supernatant
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high molecular weight isoenzyme
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in vitro transcription translation of 2 different cDNA clones encoding tyrosinase 1 and 2
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overexpression in Streptomyces antibioticus
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overproduction of apotyrosinase (TYRC) and its activator protein (ARF378) in Escherichia coli BL21(DE3)-pLysS. tyrC and arf378 are independently but not polycistronically placed under the control of a T7 promoter in a vector, pET-21a(+). His6-tagged TYRC and His6-tagged ARF378 are simultaneously overproduced in an Escherichia coli strain harboring the plasmid pET-mel2
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recombinant expression of codon-optimized CsPPO in Escherichia coli strain BL21(DE3), ectopic expression leads to the formation of inclusion bodies
recombinant expression of His-tagged enzyme in Escherichia coli BL21
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recombinant expression of His-tagged intra-melanosomal domain of wild-type tyrosinase and temperature-sensitive OCA1-related mutant R422Q and in whole-insect Trichoplusia ni via transfection with baculovius
single gene
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the cDNAs encoding three red clover PPOs are expressed individually in alfalfa (Medicago sativa L.)
the cDNAs encoding three Trifolium pratensePPOs are expressed individually in alfalfa (Medicago sativa L.)
the wild-type sequence coding for the enzyme is obtained by restriction digestion of the entire cDNA sequence, subcloning in Escherichia coli H10Bac, and recombinant expression of His-tagged enzyme in Spdoptera frugiperda Sf9 cells via the baculovirus transfection method
the wild-type Tyr and mutant constructs are transiently expressed in human embryonic kidney (HEK) 293T cells for functional analysis
two distinct messages for the squid tyrosinase, represented as cDNA-1 and cDNA-2 are expressed in the ink sac. The subunit of ST94 is synthesized as a premature protein with 625 amino acid residues and an 18-residue signal sequence regionis eliminated to form the mature subunit comprised of 607 amino acid residues with a deduced molecular mass of 68993 Da
tyrosinase gene transfection, by calcium-phosphate precipitation method, into HEK293 cell significantly down-regulates the expression levels of solute carrier family 12, member 4 and 7, which are known to be Cl- transporters. Observation of significant pigmentation of the HEK293 cells
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