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1.14.15.8: steroid 15beta-monooxygenase

This is an abbreviated version!
For detailed information about steroid 15beta-monooxygenase, go to the full flat file.

Word Map on EC 1.14.15.8

Reaction

progesterone
+
reduced ferredoxin
+
O2
+
H+
=
15beta-hydroxyprogesterone
+
oxidized ferredoxin
+
H2O

Synonyms

15beta-hydroxylase, 15beta-hydroxylase CYP106A2, BmCYP106A2, Cyp106A1, CYP106A2, cytochrome P-450meg, cytochrome P450meg, steroid-15-beta-monooxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.15.8 steroid 15beta-monooxygenase

Reference

Reference on EC 1.14.15.8 - steroid 15beta-monooxygenase

Please use the Reference Search for a specific query.
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Virus, C.; Bernhardt, R.
Molecular evolution of a steroid hydroxylating cytochrome P450 using a versatile steroid detection system for screening
Lipids
43
1133-1141
2008
Priestia megaterium (Q06069)
Manually annotated by BRENDA team
Simgen, B.; Contzen, J.; Schwarzer, R.; Bernhardt, R.; Jung, C.
Substrate binding to 15beta-hydroxylase (CYP106A2) probed by FT infrared spectroscopic studies of the iron ligand CO stretch vibration
Biochem. Biophys. Res. Commun.
269
737-742
2000
Priestia megaterium (Q06069)
Manually annotated by BRENDA team
Lisurek, M.; Kang, M.J.; Hartmann, R.W.; Bernhardt, R.
Identification of monohydroxy progesterones produced by CYP106A2 using comparative HPLC and electrospray ionisation collision-induced dissociation mass spectrometry
Biochem. Biophys. Res. Commun.
319
677-682
2004
Priestia megaterium (Q06069)
Manually annotated by BRENDA team
Virus, C.; Lisurek, M.; Simgen, B.; Hannemann, F.; Bernhardt, R.
Function and engineering of the 15beta-hydroxylase CYP106A2
Biochem. Soc. Trans.
34
1215-1218
2006
Priestia megaterium (Q06069)
Manually annotated by BRENDA team
Goni, G.; Zöllner, A.; Lisurek, M.; Velazquez-Campoy, A.; Pinto, S.; Gomez-Moreno, C.; Hannemann, F.; Bernhardt, R.; Medina, M.
Cyanobacterial electron carrier proteins as electron donors to CYP106A2 from Bacillus megaterium ATCC 13368
Biochim. Biophys. Acta
1794
1635-1642
2009
Priestia megaterium (Q06069), Priestia megaterium
Manually annotated by BRENDA team
Zehentgruber, D.; Hannemann, F.; Bleif, S.; Bernhardt, R.; Lütz, S.
Towards preparative scale steroid hydroxylation with cytochrome P450 monooxygenase CYP106A2.
Chembiochem
11
713-721
2010
Priestia megaterium (Q06069)
Manually annotated by BRENDA team
Lisurek, M.; Simgen, B.; Antes, I.; Bernhardt, R.
Theoretical and experimental evaluation of a CYP106A2 low homology model and production of mutants with changed activity and selectivity of hydroxylation
Chembiochem
16
1439-1449
2008
Priestia megaterium (Q06069)
Manually annotated by BRENDA team
Berg, A.; Gustafsson, J.A.; Ingelman-Sundberg, M.
Characterization of a cytochrome P-450-dependent steroid hydroxylase system present in Bacillus megaterium
J. Biol. Chem.
251
2831-2838
1976
Priestia megaterium
Manually annotated by BRENDA team
Berg, A.; Ingelman-Sundberg, M.; Gustafsson, M.
Purification and characterization of cytochrome P-450meg
J. Biol. Chem.
254
5264-5271
1979
Priestia megaterium (Q06069)
Manually annotated by BRENDA team
Ruijssenaars, H.J.; Sperling, E.M.; Wiegerinck, P.H.; Brands, F.T.; Wery, J.; de Bont, J.A.
Testosterone 15beta-hydroxylation by solvent tolerant Pseudomonas putida S12
J. Biotechnol.
131
205-208
2007
Priestia megaterium
Manually annotated by BRENDA team
Rauschenbach, R.; Isernhagen, M.; Noeske-Jungblut, C.; Boidol, W.; Siewert, G.
Cloning sequencing and expression of the gene for cytochrome P450meg, the steroid-15 beta-monooxygenase from Bacillus megaterium ATCC 13368
Mol. Gen. Genet.
241
170-176
1993
Priestia megaterium (Q06069)
Manually annotated by BRENDA team
Bleif, S.; Hannemann, F.; Lisurek, M.; Von Kries, J.; Zapp, J.; Dietzen, M.; Antes, I.; Bernhardt, R.
Identification of CYP106A2 as a regioselective allylic bacterial diterpene hydroxylase
ChemBioChem
12
576-582
2011
Priestia megaterium (Q06069), Priestia megaterium ATCC 13368 (Q06069)
Manually annotated by BRENDA team
Bleif, S.; Hannemann, F.; Zapp, J.; Hartmann, D.; Jauch, J.; Bernhardt, R.
A new Bacillus megaterium whole-cell catalyst for the hydroxylation of the pentacyclic triterpene 11-keto-beta-boswellic acid (KBA) based on a recombinant cytochrome P450 system
Appl. Microbiol. Biotechnol.
93
1135-1146
2012
Priestia megaterium, Priestia megaterium ATCC 13368
Manually annotated by BRENDA team
Schumacher, S.D.; Hannemann, F.; Teese, M.G.; Bernhardt, R.; Jose, J.
Autodisplay of functional CYP106A2 in Escherichia coli
J. Biotechnol.
161
104-112
2012
Priestia megaterium
Manually annotated by BRENDA team
Kiss, F.M.; Schmitz, D.; Zapp, J.; Dier, T.K.; Volmer, D.A.; Bernhardt, R.
Comparison of CYP106A1 and CYP106A2 from Bacillus megaterium - identification of a novel 11-oxidase activity
Appl. Microbiol. Biotechnol.
99
8495-8514
2015
Priestia megaterium, Priestia megaterium (Q06069)
Manually annotated by BRENDA team
Janocha, S.; Carius, Y.; Hutter, M.; Lancaster, C.R.; Bernhardt, R.
Crystal structure of CYP106A2 in substrate-free and substrate-bound form
ChemBioChem
17
852-860
2016
Priestia megaterium (Q06069)
Manually annotated by BRENDA team
Kim, K.H.; Lee, C.W.; Dangi, B.; Park, S.H.; Park, H.; Oh, T.J.; Lee, J.H.
Crystal structure and functional characterization of a cytochrome P450 (BaCYP106A2) from Bacillus sp. PAMC 23377
J. Microbiol. Biotechnol.
27
1472-1482
2017
Bacillus sp. PAMC 23377
Manually annotated by BRENDA team
Schmitz, D.; Zapp, J.; Bernhardt, R.
Steroid conversion with CYP106A2 - production of pharmaceutically interesting DHEA metabolites
Microb. Cell Fact.
13
81
2014
Priestia megaterium (Q06069)
Manually annotated by BRENDA team
Nikolaus, J.; Nguyen, K.T.; Virus, C.; Riehm, J.L.; Hutter, M.; Bernhardt, R.
Engineering of CYP106A2 for steroid 9alpha- and 6beta-hydroxylation
Steroids
120
41-48
2017
Priestia megaterium (Q06069)
Manually annotated by BRENDA team