Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.14.15.1: camphor 5-monooxygenase

This is an abbreviated version!
For detailed information about camphor 5-monooxygenase, go to the full flat file.

Word Map on EC 1.14.15.1

Reaction

(+)-Camphor
+
reduced putidaredoxin
+
O2
=
(+)-exo-5-hydroxycamphor
+
oxidized putidaredoxin
+
H2O

Synonyms

2-bornanone 5-exo-hydroxylase, bornanone 5-exo-hydroxylase, CamC, camphor 5-exo-hydroxylase, camphor 5-exo-methylene hydroxylase, camphor 5-exohydroxylase, camphor 5-hydroxylase, Camphor 5-monooxygenase, camphor hydroxylase, camphor hydroxylase cytochrome P450cam, camphor methylene hydroxylase, camphor monooxygenase, class I cytochrome P450, CYP101, CYP101A1, CYP101B1, CYP101C1, CYP101D1, CYP101D2, CYP111A2, Cyt P450cam, cytochrome P-450-CAM, cytochrome P450 cam, cytochrome P450(cam), cytochrome p450cam, cytochrome P450cam monooxygenase, d-camphor monooxygenase, D-camphor-exo-hydroxylase, haem mono-oxygenase CYP101, methylene hydroxylase, methylene monooxygenase, moe, oxygenase, camphor 5-mono-, P450cam, P450cam monooxygenase, P450tcu

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.15.1 camphor 5-monooxygenase

Storage Stability

Storage Stability on EC 1.14.15.1 - camphor 5-monooxygenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-196°C, no loss of activity of putidaredoxin reductase after repeated freeze-thaw cycles
-
-20°C, 50 mM Tris buffer, pH 7.4, 50% v/v glycerol
-
-80°C, 50 mM potassium phosphate buffer, pH 7.4, 50 mM KCl, 1 mM (+)-camphor
0-4°C, after 24-96 h, the resuspended pellet of 140000*g sedimentation of Pseudomonas sp. C5 loses hydroxylation capacity, restorable with NADPH and THF
-
0°C, putidaredoxin slowly loses its prosthetic group, 2-mercaptoethanol retards apoprotein formation
-
4°C, monomeric cytochrome P450cam can be stored at low protein concentrations for several days without appreciable accumulation of the dimer
-