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1.14.15.1: camphor 5-monooxygenase

This is an abbreviated version!
For detailed information about camphor 5-monooxygenase, go to the full flat file.

Word Map on EC 1.14.15.1

Reaction

(+)-Camphor
+
reduced putidaredoxin
+
O2
=
(+)-exo-5-hydroxycamphor
+
oxidized putidaredoxin
+
H2O

Synonyms

2-bornanone 5-exo-hydroxylase, bornanone 5-exo-hydroxylase, CamC, camphor 5-exo-hydroxylase, camphor 5-exo-methylene hydroxylase, camphor 5-exohydroxylase, camphor 5-hydroxylase, Camphor 5-monooxygenase, camphor hydroxylase, camphor hydroxylase cytochrome P450cam, camphor methylene hydroxylase, camphor monooxygenase, class I cytochrome P450, CYP101, CYP101A1, CYP101B1, CYP101C1, CYP101D1, CYP101D2, CYP111A2, Cyt P450cam, cytochrome P-450-CAM, cytochrome P450 cam, cytochrome P450(cam), cytochrome p450cam, cytochrome P450cam monooxygenase, d-camphor monooxygenase, D-camphor-exo-hydroxylase, haem mono-oxygenase CYP101, methylene hydroxylase, methylene monooxygenase, moe, oxygenase, camphor 5-mono-, P450cam, P450cam monooxygenase, P450tcu

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.15.1 camphor 5-monooxygenase

Cloned

Cloned on EC 1.14.15.1 - camphor 5-monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
ArR/Arx electron-transport chain co-expressed with the CYP enzymes in Escherichia coli. Amplified genes incorporated into the pRSFDuet-Arx vector using the Nde I and Kpn I/EcoR V restriction sites, plasmids pETDuet-Arx-ArR and one of the P450 expressing plasmids pRSFDuet-Arx-CYP transformed into Escherichia coli competent BL21(DE3) cells
-
expressed in Escherichia coli strain JM109
expression in Escherichia coli
expression of C334A enzyme mutant in Escherichia coli strain NCM533
-
expression of enzyme and cofactor, separately, in Escherichia coli strain DH5alpha
-
expression of enzyme and mutant cofactor in Escherichia coli strain BL21(DE3)
-
expression of N-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
expression of P450cam C334A in Escherichia coli NCM533
expression of wild-type and mutant enzymes in Escherichia coli
expression of wild-type enzyme, encoded by gene camC, with gene camB, encoding the putidaredoxin, in Escherichia coli strains BL21(DE3), expression of mutant enzymes in Escherichia coli strain DH10B
-
exxpression of N-terminally His-tagged CYP101D2 in Escherichia coli strain BL21(DE3)
functional co-expression with glycerol dehydrogenase in Escherichia coli strain BL21(DE3)
-
functional expression in Escherichia coli DH5alpha of tricistronic constructs consisting of P450cam encoded by the first cistron and the auxiliary proteins, putidaredoxin and putidaredoxin reductase by the second and the third
-
gene camC, expression of mutant enzymes in Escherichia coli strain BL21(DE3)
gene camC, recombinant expression of wild-type enzyme and chimeric enzyme fusion protein P450cam-PdR in Escherichia coli strain BL21 (DE3)
individual expression of enzyme and cofactor putidaredoxin in Escherichia coli
-
mutant C334A transformed into chemically competent Escherichia coli NCM533 cells
-
mutants overexpressed from Escherichia coli strain NCM533 harboring modified pDNC334A plasmids that encode the appropriate mutant of CYP101 under control of the lac promoter
-
open reading frames for P450cam mutants including a 6 x His carboxyl-terminal tag cloned into the pCW(Ori+) expression vector using the NdeI and XbaI restriction sites, expressed in Escherichia coli
overexpression in Escherichia coli
-
overexpression in Escherichia coli strain BL21(DE3)
overexpression of P450cam mutant C334A in Escherichia coli strain BL21(DE3)
overexpression of wild-type and mutant enzyme sin Escherichia coli
-
pCHC1 plasmid, encoding the wild type cytochrome P450cam (C334A mutant of the native enzyme). PCR products transformed into Escherichia coli XL1-blue super-competent cells. Wild-type and mutants expressed in Escherichia coli BL21 (DE3) cells
plasmids transformed into Escherichia coli Bl21(DE3) competent cells
-
Pseudomonas putida, cam operon has been isolated, cloned and expressed in Escherichia coli, review
-
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain XL-1 Blue from expression plasmids, pSUABC, under control of the salicylate promoter
recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
recombinant expression of wild-type and mutant enzymes in Escherichia coli, the selenoenzyme mutant SeP450cam C357U/R365L/E366Q is expressed in Escherichia coli strain XL1 blue cotransformed with plasmid pSUABC, whereas mutant R365L/E366Q P450cam and wild-type P450cam are expressed in a Escherichia coli strain BL21 Gold, which enhances the yields of the latter proteins but not of SeP450cam
recombinant expression of wild-type enzyme, mutant P450cam[Tyr96Phe]-RhFRed, and other enzyme mutants in Escherichia coli strain BL21(DE3)
the enzyme is CAM plasmid encoded
-