1.14.14.9: 4-hydroxyphenylacetate 3-monooxygenase

This is an abbreviated version!
For detailed information about 4-hydroxyphenylacetate 3-monooxygenase, go to the full flat file.

Word Map on EC 1.14.14.9

1.14.14.9

flavin

3,4-dihydroxyphenylacetate

baumannii

flavin-dependent

tyrosol

hydroxytyrosol

fmnh

two-protein

piceatannol

synthesis

Reaction

Synonyms

4 HPA 3-hydroxyylase, 4-HPA hydroxylase, 4-hydroxyphenylacetate 3-hydroxylase, 4-hydroxyphenylacetic acid 3-hydroxylase, 4HPA 3-monooxygenase, 4HPA3H, C2-hpah, EC 1.14.13.3, HPA 3-hydroxylase, HpaB, hpaBC, HpaC, HPAH, More, p-hydroxyphenylacetate 3-hydroxylase, p-hydroxyphenylacetate hydroxylase, p-hydroxyphenylacetic 3-hydroxylase, TPY_2462, two-component p-hydroxyphenylacetate hydroxylase

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

1.14.14.9 4-hydroxyphenylacetate 3-monooxygenase

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Results	in table
4	Activating Compound
1749	AA Sequence
7	Application
1	CAS Registry Number
20	Cloned(Commentary)
72	Cofactor
8	Crystallization (Commentary)
2	General Information
3	General Stability
21	Inhibitors
31	kcat/KM [mM/s]
57	KM Value [mM]
2	Localization
5	Metals/Ions
24	Molecular Weight [Da]
49	Natural Substrates/ Products (Substrates)
367	Organism
7	Pathway
12	PDB ID
21	pH Optimum
4	pH Range
49	Protein Variants
17	Purification (Commentary)
20	Reaction
4	Reaction Type
71	Reference
1	Source Tissue
20	Specific Activity [micromol/min/mg]
4	Storage Stability
187	Substrates and Products (Substrate)
28	Subunits
70	Synonyms
1	Systematic Name
16	Temperature Optimum [°C]
1	Temperature Stability [°C]
34	Turnover Number [1/s]

Inhibitors

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Inhibitors on EC 1.14.14.9 - 4-hydroxyphenylacetate 3-monooxygenase

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4-hydroxyphenylacetate

Cibacron blue F3GA

Co2+

Cu2+

EDTA

inhibits non-purified enzyme, ammonium sulfate precipitate

288040

FAD

Acinetobacter baumannii

inhibitory effect at more than 0.15 mM

438900

FMN

Acinetobacter baumannii

inhibitory effect at more than 0.15 mM

Hg2+

iodoacetate

N-ethylmaleimide

o-phenanthroline

complete inhibition at 1 mM

438886

p-chloromercuribenzoate

Pseudomonas putida

92% inhibition at 1 mM

438887

p-coumaric acid

Pseudomonas aeruginosa

Q9HWT7; Q9HWT6

substrate inhibition at concentrations higher than 40 mM

744140

Zn2+

Pseudomonas putida

438895

additional information

2 entries