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1.14.14.33: ethylenediaminetetraacetate monooxygenase

This is an abbreviated version!
For detailed information about ethylenediaminetetraacetate monooxygenase, go to the full flat file.

Reaction

ethylenediaminetetraacetate
+ 2 FMNH2 + 2 O2 =
ethylenediamine-N,N'-diacetate
+ 2 glyoxylate + 2 FMN + 2 H2O

Synonyms

EmoA

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.33 ethylenediaminetetraacetate monooxygenase

Crystallization

Crystallization on EC 1.14.14.33 - ethylenediaminetetraacetate monooxygenase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
to 2.15 A resolution. The structure shows a domain-like insertion into a TIM-barrel, which might serve as a flexible lid for the active site. Docking of MgEDTA2- into EmoA identifies an intricate hydrogen bond network connected to Tyr71, which should lower its pKa. Tyr71, along with nearby Glu70 and a peroxy flavin, facilitates a keto-enol transition of the leaving acetyl group of EDTA. The interaction between EmoA and oxidoreductase EmoB enhances both EmoA and EmoB activities probably through coupled channelling of FMNH2