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1.14.14.20: phenol 2-monooxygenase (FADH2)

This is an abbreviated version!
For detailed information about phenol 2-monooxygenase (FADH2), go to the full flat file.

Word Map on EC 1.14.14.20

Reaction

phenol
+
FADH2
 +
O2
=
catechol
 +
FAD
 +
H2O

Synonyms

FAD reductase, flavin reductase PheA2, PheA, PheA1, PheA2, phenol hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.20 phenol 2-monooxygenase (FADH2)

Crystallization

Crystallization on EC 1.14.14.20 - phenol 2-monooxygenase (FADH2)

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant PheA2 containing bound FAD cofactor and purified reduced holo-PheA2 in complex with oxidized NAD, hanging drop vapor diffusion method, mixing of 0.002 ml of 16 mg/ml protein in 50 mM sodium phosphate buffer, pH 7.0, with 0.002 ml of reservoir solution containing 20-26% PEG 3350 and 0.4 M magnesium nitrate, and equilibration against 1 ml of reservoir solution, 20°C, several days, crystal soakig in NADH or FAD solution, X-ray diffraction structure determination and analysis at 2.1-2.2 A resolution, modeling
-