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1.14.13.84: 4-hydroxyacetophenone monooxygenase

This is an abbreviated version!
For detailed information about 4-hydroxyacetophenone monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.84

Reaction

(4-hydroxyphenyl)ethan-1-one
+
NADPH
 +
H+
 +
O2
=
4-hydroxyphenyl acetate
 +
NADP+
 +
H2O

Synonyms

4-hydroxyacetophenone monooxygenase, arylketone monooxygenase, Baeyer-Villiger monooxygenase, hAPA, HAPMO, More, oxygenase, 4-hydroxyacetophenone mono-

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.84 4-hydroxyacetophenone monooxygenase

Turnover Number

Turnover Number on EC 1.14.13.84 - 4-hydroxyacetophenone monooxygenase

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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
21.2
2'-hydroxyacetophenone
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
2.1 - 3.5
2,4-Pentanedione
7.8
2-acetylpyridine
-
pH 7.5, 30°C
9.4 - 11.3
2-acetylpyrrole
4.2
2-decanone
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
6.7
2-hydroxyacetophenone
-
pH 7.5, 30°C
8.7
2-phenylpropionaldehyde
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
8.6
2-pyrrole carboxaldehyde
-
pH 7.5, 30°C
8.1
3'-hydroxyacetophenone
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
1
3-aminoacetophenone
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
7.4
3-chloro-2-butanone
-
pH 7.5, 30°C
4.8
3-hydroxyacetophenone
-
pH 7.5, 30°C
0.4
3-methoxyacetophenone
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
1.2
3-nitroacetophenone
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
0.5
4-acetylpyridine
-
pH 7.5, 30°C
8.2 - 12.7
4-aminoacetophenone
1.6
4-chloroacetophenone
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
0.6 - 1.3
4-fluoroacetophenone
5.4
4-hydroxy-3-methylacetophenone
pH 8, 30°C
9.8 - 12.6
4-hydroxyacetophenone
7.6
4-hydroxybenzaldehyde
10.6 - 11.9
4-hydroxypropiophenone
1.2 - 1.7
4-methoxyacetophenone
4.4 - 6.3
4-methylacetophenone
2.6
4-Nitroacetophenone
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
4.5 - 16.3
acetophenone
3.9
acetylcyclohexane
-
pH 7.5, 30°C
2.2
benzaldehyde
6.7
bicyclohept-2-en-6-one
-
pH 7.5, 30°C
1.2 - 4.9
butyrophenone
5
cyclohexane carboxaldehyde
-
pH 7.5, 30°C
4.1
Hydroxyacetone
-
pH 7.5, 30°C
2.5
isobutyrophenone
-
pH 7.5, 30°C
7.3
methyl 4-tolyl sulfide
-
pH 7.5, 30°C
20
methyl-4-tolyl sulfide
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C
4.7
methylphenyl sulfide
-
pH 7.5, 30°C
0.19 - 5.1
NADH
0.1 - 10.1
NADPH
2.6 - 11
propiophenone
15.4
trifluoroacetophenone
312 microM NADPH, 50 mM Tris-HCl, pH 8.0, 30°C