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1.14.13.7: phenol 2-monooxygenase (NADPH)

This is an abbreviated version!
For detailed information about phenol 2-monooxygenase (NADPH), go to the full flat file.

Word Map on EC 1.14.13.7

Reaction

phenol
+
NADPH
 +
H+
 +
O2
=
catechol
 +
NADP+
 +
H2O

Synonyms

DmpLNO, flavin containing monooxygenase, LmPH, Mph, MphN, multi-component phenol hydroxylase, multicomponent PH, multicomponent phenol hydroxylase, multicomponent phenol hydroxylase alpha subunit, NCgl2588, oxygenase, phenol 2-mono-, PHE, phenol hydroxylase, phenol o-hydroxylase, PHH, phhY, PHIND, PHO, PHR, single-component PH, SPH

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.7 phenol 2-monooxygenase (NADPH)

Purification

Purification on EC 1.14.13.7 - phenol 2-monooxygenase (NADPH)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
His-bind Ni-NTA resin column chromatography
-
native enzyme partially by ammonium sulfate fractionation and dialysis, cost-effective production of partially purified microalgal phenol hydroylase by single-step purification
-
oxygenase component PHO
-
purification of the oxygenase component
-
recombinant PHK, the accessory component of the phenol hydroxylase, from Escherichia coli
-