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1.14.13.50: pentachlorophenol monooxygenase

This is an abbreviated version!
For detailed information about pentachlorophenol monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.50

Reaction

2,3,5,6-Tetrachlorophenol
+
NADPH
 +
H+
 +
O2
=
2,3,5,6-tetrachlorohydroquinone
 +
NADP+
 +
H2O

Synonyms

CpsB, dechlorinase, pentachlorophenol, GM610_18120, oxygenase, pentachlorophenol 4-mono-, PCB hydroxylase, PCP 4-monooxygenase, PCP hydroxylase, PCP-4-monooxygenase, PCP-monooxygenase, PCP4MO, PcpB, PcpD, pentachlorophenol 4-mono-oxygenase, pentachlorophenol 4-monooxygenase, pentachlorophenol dechlorinase, pentachlorophenol dehalogenase, pentachlorophenol hydroxylase, pentachlorophenol monooxygenase, pentachlorophenol-4-monooxygenase, Phe4MO, tetrachlorobenzoquinone reductase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.50 pentachlorophenol monooxygenase

Cloned

Cloned on EC 1.14.13.50 - pentachlorophenol monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
analogues of PCP catabolic genes (cpsABCDE, and p450) are successfully amplified from the whole genome of BtAOA using the primers designed based on the gene sequences retrieved from the whole genome annotation data submitted at NCBI
-
DNA and amino acid sequence determination and analysis, expression of His-tagged wild-type and mutant enzymes in Escherichia coli
-
Escherichia coli M15 overexpressing recombinant hexahistidine-tagged pentachlorophenol 4-monooxygenase
expression in Escherichia coli
-
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
gene isolated from Pseudomonas sp. plasmid, recombinant expression in Escherichia coli XLBlue1alpha, DNA sequence analysis of the recombinant clones, comparison with other sequences
-
gene pcpB, DNA and amino acid sequence analysis, phylogenetic analysis
gene pcpB, expression in Escherichia coli
-
gene pcpD, expression as C-terminally His12-tagged protein in Pseudomonas aeruginosa strain PAO1-Lac
-
pcpB gene, constitutive overexpression in Escherichia coli, DNA sequence determination and analysis
pcpB gene, expression in Escherichia coli and Flavobacterium sp. ATCC 39723 of wild-type and isogenic mutant, DNA sequence analysis
-
pcpB gene, expression in Escherichia coli BL21, DNA sequence analysis
pcpB gene, expression of wild-type and mutant in Escherichia coli as glutathione-S-transferase fusion protein
-
sequence comparisons and phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-