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1.14.13.215: protoasukamycin 4-monooxygenase

This is an abbreviated version!
For detailed information about protoasukamycin 4-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.215

Reaction

protoasukamycin
+
NADH
+
H+
+
O2
=
4-hydroxyprotoasukamycin
+
NAD+
+
H2O

Synonyms

asuE1, flavin-dependent protoasukamycin hydroxylase, protoasukamycin 4-hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.215 protoasukamycin 4-monooxygenase

Purification

Purification on EC 1.14.13.215 - protoasukamycin 4-monooxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chomatography