1.14.12.12: naphthalene 1,2-dioxygenase

This is an abbreviated version!
For detailed information about naphthalene 1,2-dioxygenase, go to the full flat file.

Word Map on EC 1.14.12.12

1.14.12.12

putida

polycyclic

bioremediation

rieske

toluene

phenanthrene

dioxygenation

naphthalene-degrading

ring-hydroxylating

dihydroxylation

indigo

pah-degrading

hydrocarbon-degrading

dihydrodiols

cis-dihydrodiols

rieske-type

oil-contaminated

cis-dihydroxylation

parales

2-nitrotoluene

pah-contaminated

acenaphthene

cis-diols

side-on

alpha3beta3

indene

synthesis

Reaction

Naphthalene

+

NADH

+

H+

+

O2

=

(1R,2S)-1,2-dihydronaphthalene-1,2-diol

+

NAD⁺

Synonyms

NAG, NAHA, nahAc, naphthalene 1,2-dioxygenase, naphthalene dioxygenase, naphthalene oxygenase, NDO, oxygenase, naphthalene di-

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.12 With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor

1.14.12.12 naphthalene 1,2-dioxygenase

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Results	in table
1	Activating Compound
153	AA Sequence
1	Application
1	CAS Registry Number
15	Cloned(Commentary)
44	Cofactor
7	Crystallization (Commentary)
2	General Information
8	General Stability
6	Inhibitors
5	KM Value [mM]
5	Localization
13	Metals/Ions
24	Molecular Weight [Da]
21	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
113	Organism
6	Pathway
70	PDB ID
5	pH Optimum
43	Protein Variants
15	Purification (Commentary)
5	Reaction
5	Reaction Type
77	Reference
5	Specific Activity [micromol/min/mg]
3	Storage Stability
224	Substrates and Products (Substrate)
16	Subunits
38	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
2	Temperature Stability [°C]
2	Turnover Number [1/s]

Molecular Weight

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Molecular Weight on EC 1.14.12.12 - naphthalene 1,2-dioxygenase

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13500

Sphingomonas sp.

-

ferredoxin component, gel filtration

155000

Rhodococcus sp.

-

terminal dioxygenase ISPNAR, gel filtration

158000

Pseudomonas sp.

-

component B, i.e. oxygenase ISPNAP, gel filtration

20000

show

200000

Sphingomonas sp.

-

oxydase component, gel filtration

23000

Rhodococcus sp.

-

alpha2beta2, 2 * 55000 + 2 * 23000, SDS-PAGE

25000

Pseudomonas putida

-

x * 53000 + x * 25000, histidine-tagged recombinant enzyme, SDS-PAGE

34900

Pseudomonas sp.

-

ferredoxinNAP reductase, native PAGE

36000

Pseudomonas sp.

-

1 * 36000, ferredoxinNAP reductase, SDS-PAGE

37000

show

37100

Pseudomonas sp.

-

ferredoxinNAP reductase, deduced from amino acid sequence

43000

Corynebacterium renale

-

1 * 43000 + 1 * 56000, SDS-PAGE

45000

Sphingomonas sp.

-

reductase component, gel filtration

52000

Sphingomonas sp.

-

3 * 52000, 3 * 20000, alpha3beta3 hexamer, oxygenase component, SDS-PAGE

53000

Pseudomonas putida

-

x * 53000 + x * 25000, histidine-tagged recombinant enzyme, SDS-PAGE

55000

show

56000

Corynebacterium renale

-

1 * 43000 + 1 * 56000, SDS-PAGE

99000

Corynebacterium renale

-

gel filtration

additional information

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Release 2023.1 (January 2023)