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1.14.12.12: naphthalene 1,2-dioxygenase

This is an abbreviated version!
For detailed information about naphthalene 1,2-dioxygenase, go to the full flat file.

Word Map on EC 1.14.12.12

Reaction

Naphthalene
+
NADH
 +
H+
 +
O2
=
(1R,2S)-1,2-dihydronaphthalene-1,2-diol
 +
NAD+

Synonyms

NAG, NAHA, nahAc, naphthalene 1,2-dioxygenase, naphthalene dioxygenase, naphthalene oxygenase, NDO, oxygenase, naphthalene di-

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.12 With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
                1.14.12.12 naphthalene 1,2-dioxygenase

Engineering

Engineering on EC 1.14.12.12 - naphthalene 1,2-dioxygenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D362A
-
mutation in alpha subunit of terminal dioxygenase ISPNAP, no activity with naphthalene, biphenyl and phenanthrene
F202L
-
mutation in alpha subunit of terminal dioxygenase ISPNAP, no activity with naphthalene, biphenyl and phenanthrene
F202V
-
mutation in alpha subunit of terminal dioxygenase ISPNAP, similar activity with naphthalene, biphenyl and phenanthrene as wild-type
F224A
-
6.8% of wild-type activity with substrate naphthalene
F224C
-
9.1% of wild-type activity with substrate naphthalene
F224D
-
85.2% of wild-type activity with substrate naphthalene
F224E
-
94.7% of wild-type activity with substrate naphthalene
F224G
-
2.1% of wild-type activity with substrate naphthalene
F224H
-
92% of wild-type activity with substrate naphthalene
F224I
-
41.9% of wild-type activity with substrate naphthalene
F224K
-
95.3% of wild-type activity with substrate naphthalene
F224L
-
92.58% of wild-type activity with substrate naphthalene
F224M
-
97.5% of wild-type activity with substrate naphthalene
F224N
-
94.7% of wild-type activity with substrate naphthalene
F224P
-
15.1% of wild-type activity with substrate naphthalene
F224Q
-
20.4% of wild-type activity with substrate naphthalene
F224R
-
0.7% of wild-type activity with substrate naphthalene
F224S
-
2% of wild-type activity with substrate naphthalene
F224T
-
4.9% of wild-type activity with substrate naphthalene
F224V
-
13.2% of wild-type activity with substrate naphthalene
F224W
-
91.8% of wild-type activity with substrate naphthalene
F224Y
-
97% of wild-type activity with substrate naphthalene
F352L
-
mutation in alpha subunit of terminal dioxygenase ISPNAP, similar activity with naphthalene, biphenyl and phenanthrene as wild-type, major product of biphenyl oxidation: biphenyl-3,4-dihydrodiol instead of biphenyl-2,3-dihydrodiol
F352V
M366W
-
mutation in alpha subunit of terminal dioxygenase ISPNAP, similar activity with naphthalene, biphenyl and phenanthrene as wild-type
N201A
-
mutation in alpha subunit of terminal dioxygenase ISPNAP, similar activity with naphthalene as wild-type, very weak activity with biphenyl
N201Q
-
mutation in alpha subunit of terminal dioxygenase ISPNAP, similar activity with naphthalene, biphenyl and phenanthrene as wild-type
N201S
-
mutation in alpha subunit of terminal dioxygenase ISPNAP, similar activity with naphthalene, biphenyl and phenanthrene as wild-type
T351N
-
mutation in alpha subunit of terminal dioxygenase ISPNAP, similar activity with naphthalene, biphenyl and phenanthrene as wild-type
T351R
-
mutation in alpha subunit of terminal dioxygenase ISPNAP, similar activity with naphthalene, biphenyl and phenanthrene as wild-type
T351S
-
mutation in alpha subunit of terminal dioxygenase ISPNAP, similar activity with naphthalene, biphenyl and phenanthrene as wild-type
V260A
-
mutation in alpha subunit of terminal dioxygenase ISPNAP, similar activity with naphthalene, biphenyl and phenanthrene as wild-type
V260L
-
mutation in alpha subunit of terminal dioxygenase ISPNAP, similar activity with naphthalene, biphenyl and phenanthrene as wild-type
V260N
-
mutation in alpha subunit of terminal dioxygenase ISPNAP, similar activity with naphthalene, biphenyl and phenanthrene as wild-type
W316A
-
mutation in alpha subunit of terminal dioxygenase ISPNAP, similar activity with naphthalene, biphenyl and phenanthrene as wild-type
W358A
-
mutation in alpha subunit of terminal dioxygenase ISPNAP, very low activity with naphthalene and biphenyl
F224A
-
6.8% of wild-type activity with substrate naphthalene
-
F224C
-
9.1% of wild-type activity with substrate naphthalene
-
F224D
-
85.2% of wild-type activity with substrate naphthalene
-
F224S
-
2% of wild-type activity with substrate naphthalene
-
F224Y
-
97% of wild-type activity with substrate naphthalene
-
F352V
-
mutation changes the product regioselectivities for naphthalene, phenanthrene, and biphenyl
-