1.14.11.56: L-proline cis-4-hydroxylase
This is an abbreviated version!
For detailed information about L-proline cis-4-hydroxylase, go to the full flat file.
Word Map on EC 1.14.11.56
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1.14.11.56
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cis-4-hydroxy-l-proline
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sinorhizobium
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meliloti
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dioxygenase
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hydroxylases
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regioselectivity
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loti
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chop
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non-heme
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mesorhizobium
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synthesis
- 1.14.11.56
- cis-4-hydroxy-l-proline
-
sinorhizobium
- meliloti
- dioxygenase
- hydroxylases
-
regioselectivity
- loti
-
chop
-
non-heme
-
mesorhizobium
- synthesis
Reaction
Synonyms
cis-P4H, L-proline cis-4-hydroxylase, MlP4H, mlr6283, R03107, SmP4H
ECTree
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Crystallization
Crystallization on EC 1.14.11.56 - L-proline cis-4-hydroxylase
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in complex with in complex with Co2+, 2-oxoglutarate as cofactors, and L-proline or L-pipecolic acid. The active site is composed of a distorted jelly roll beta-sheet core, which is sandwiched by the N-terminal and C-terminal alpha-helical domains. Co2+ is coordinated by residues H106, H154, and D108
purified recombinant enzyme MlP4H in complex with Co2+, 2-oxoglutarate and L-Pro or L-Pip, the MlP4H protein used for crystallization includes an extra (Met)-Ser-Ala-Trp-Ser-His-Pro-Gln-Phe-Gly-Lys-Gly-Ala strep-tag II peptide at its N-terminus, and the original start codon of the wild-type is replaced by the underlined alanine, followed by the second codon of the wild-type. Crystallization of L-Pro complex crystals by sitting-drop vapor diffusion method mixing 0.001 ml of 28 mg/ml protein solution containing 2 mM CoCl2, 10 mM 2-oxoglutarate, and 20 mM L-Pro with reservoir solution containing 0.1 M bis-Tris propane, pH 8.5, 0.2 M sodium malonate, and 25% v/v PEG 3350, or of L-Pip complex crystals by sitting drop vapour diffusion method mixing 0.001 ml of 28 mg/ml protein solution containing 2 mM CoCl2, 10 mM ?2-oxoglutarate, and 20 mM L-Pip with 0.001 ml of reservoir solution containing 0.1 M CAPS, pH 10.5, 0.1 M lithium sulfate, and 1.8 M ammonium sulfate, all at 15°C, X-ray diffraction structure determination and analysis at 1.3-2.8 A resolution, by single-wavelength dispersion method with the bound Co2+ at the active site used as the anomalous scatter or by molecular replacement using the first L-Pro-bound structure as a search model