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1.14.11.33: DNA oxidative demethylase

This is an abbreviated version!
For detailed information about DNA oxidative demethylase, go to the full flat file.

Word Map on EC 1.14.11.33

Reaction

DNA-base-CH3
+
2-oxoglutarate
+
O2
=
DNA-base
+
formaldehyde
+
succinate
+
CO2

Synonyms

1-methyladenine-DNA dioxygenase, ABH1, ABH2, ABH3, AlkB, AlkB homolog 1, ALKBH2, ALKBH3, ALKBH8, alkylated DNA repair protein, alpha-ketoglutarate-dependent dioxygenase ABH1, FTO

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.33 DNA oxidative demethylase

Cloned

Cloned on EC 1.14.11.33 - DNA oxidative demethylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AlkB is expressed in Escherichia coli BL21(DE3) cells
-
AlkB overexpression in Escherichia coli AlkB-deficient strains HK82 and BW25113. AlkB mutant strain HK82 is transformed with the low-copy-number expression plasmid pJB658 containing the alkB gene following a toluic acid-inducible promoter. The recombinant AlkB expressed is less active than the natural endogenous form
-
expressed in Escherichia BL21-CodonPlus(DE3)-RIPL cells
expressed in Escherichia coli B834lambda (DE3) cells
-
expressed in Escherichia coli BL21 Star(DE3)pLysS cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) cells and in Sf9 insect cells
expressed in Escherichia coli BL21(DE3) cells and in Sf9 insect cells. ABH3 expressed in insect cells contains no posttranslational modifications but is nevertheless found to be 5fold more active than ABH3 purified from Escherichia coli, suggesting ABH3 may not be correctly folded when purified from Escherichia coli
-
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL cells
expressed in Escherichia coli CodonPlus RIPL cells
-
expression of GFP-tagged enzyme in HeLa cells, localization in nucleoli due to the genuine nucleolar localization signal sequence. Ectopic expression of wild-type ABH2 leads to a 2.5fold increase of pre-rRNA, whereas ectopic expression of D173A mutant only leads to a marginal increase of pre-rRNA
-
isoforms ABH2 and ABH3 are expressed in baculovirus-infected Sf9 insect cells
-
isoforms ABH2 and ABH3 are expressed in Sf9 insect cells
-