1.14.11.33: DNA oxidative demethylase

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For detailed information about DNA oxidative demethylase, go to the full flat file.

Reaction

Synonyms

1-methyladenine-DNA dioxygenase, ABH1, ABH2, ABH3, AlkB, AlkB homolog 1, ALKBH2, ALKBH3, ALKBH8, alkylated DNA repair protein, alpha-ketoglutarate-dependent dioxygenase ABH1, FTO

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor

                1.14.11.33 DNA oxidative demethylase

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Results	in table
1	Activating Compound
1770	AA Sequence
1	Application
28	Cloned(Commentary)
28	Cofactor
1	Crystallization (Commentary)
188	Disease/ Diagnostics
26	General Information
1	IC50 Value
3	Inhibitors
11	kcat/KM [mM/s]
14	KM Value [mM]
8	Localization
41	Metals/Ions
53	Natural Substrates/ Products (Substrates)
105	Organism
20	PDB ID
6	pH Optimum
32	Protein Variants
24	Purification (Commentary)
1	Reaction
16	Reaction Type
54	Reference
12	Source Tissue
1	Storage Stability
256	Substrates and Products (Substrate)
82	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
13	Turnover Number [1/s]

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Application on EC 1.14.11.33 - DNA oxidative demethylase

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APPLICATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

analysis

Homo sapiens

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development of a fluorogenic probe design (MAQ) that is directly responsive to ALKBH3 repair activity. MAQ uses the fluorescence-quenching properties of 1-methyladenine, removal of the alkyl group results in an more than 10fold light-up signal. The probe is specific for ALKBH3 over its related homologue ALKBH2 and can be used to identify and measure the effectiveness of enzyme inhibitors. The probe functions efficiently in cells, allowing imaging and quantitation of ALKBH3 activity by microscopy and flow cytometry

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