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1.13.12.7: firefly luciferase

This is an abbreviated version!
For detailed information about firefly luciferase, go to the full flat file.

Word Map on EC 1.13.12.7

Reaction

D-firefly luciferin
+
O2
 +
ATP
=
firefly oxyluciferin
 +
CO2
 +
AMP
 +
diphosphate
 +
hnu

Synonyms

AL1, AL2, beetle luciferase, CBG99luc, CBRluc, FFL, firefly luciferase, firefly luciferin luciferase, fluc, LpLuc1, LpLuc2, Luc, Luc1, Luc1-type luciferase, Luc2, Luc2-type luciferase, luciferase, luciferase (firefly luciferin), luciferase FM, luciferin, Luciola italica luciferase, lucPpe, lucPpy, orange light-producing luciferase, oxygen 4-oxidoreductase, PC3-Luc, Photinus luciferin 4-monooxygenase (ATP-hydrolyzing), Photinus pyralis luciferase, PML, PpLase, Ppy, Ppy GR-TS, Ppy RE-TS, PpyWT, PsntWT

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
                1.13.12.7 firefly luciferase

Storage Stability

Storage Stability on EC 1.13.12.7 - firefly luciferase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 20% loss of activity after 2 weeks
-
4°C, 50 mM Tris-HCl (pH 7.0) containing 150 mM NaCl, 1 mM EDTA, and 1 mM dithiothreitol, up to 6 months, 10% loss of activity
4°C, in 50 mM Tris-HCl, 1 mM EDTA, 150 mM NaCl, 1 mM DTT and 0.8 M ammonium sulfate pH 8.0
-
4°C, pH 7.0, 0.1 M phosphate, 1 mM 2-mercaptoethanol, immobilized, 3 weeks
-
4°C, pH 7.5, ammonium sulfate, EDTA, as 10 mg protein/ml slurry, several months
-
4°C, wild-type and mutant enzymes remain fully active for up to 6 months
-
5°C, pH 7.2, ammonium sulfate, EDTA, glycerol, several weeks