1.13.12.16: nitronate monooxygenase

This is an abbreviated version!
For detailed information about nitronate monooxygenase, go to the full flat file.

Reaction

Synonyms

2-nitropropane dioxygenase, EC 1.13.11.32, NAO, ncd2, nitroalkane oxidase, nitroalkane-oxidizing enzyme, nitronate monooxygenase, nitropropane dioxygenase, NMO, NMO-Nc, NMO-Ws, nmoA, Npd1, Npd2, Npd3, Npd4, Npd5, Npd6, oxidase, nitroalkane, oxygenase, 2-nitropropane di-, P3N monooxygenase, Pa-NMO, PA4202, protein PA4202, Rv1894c, Sa-NAO

ECTree

     1 Oxidoreductases

         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)

                1.13.12.16 nitronate monooxygenase

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Results	in table
3	Activating Compound
3508	AA Sequence
2	CAS Registry Number
13	Cloned(Commentary)
32	Cofactor
5	Crystallization (Commentary)
3	Expression
8	General Information
3	General Stability
84	Inhibitors
57	kcat/KM [mM/s]
20	Ki Value [mM]
122	KM Value [mM]
9	Metals/Ions
16	Molecular Weight [Da]
20	Natural Substrates/ Products (Substrates)
57	Organism
3	Pathway
16	PDB ID
26	pH Optimum
5	pH Range
1	pH Stability
11	Protein Variants
21	Purification (Commentary)
11	Reaction
3	Reaction Type
40	Reference
4	Source Tissue
9	Specific Activity [micromol/min/mg]
2	Storage Stability
234	Substrates and Products (Substrate)
14	Subunits
76	Synonyms
1	Systematic Name
21	Temperature Optimum [°C]
3	Temperature Range [°C]
6	Temperature Stability [°C]
67	Turnover Number [1/s]

KCat KM Value

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KCat KM Value on EC 1.13.12.16 - nitronate monooxygenase

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kcat/KM VALUE [1/mMs^-1]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.35

1-nitrobutane

Neurospora crassa

Q01284

in 50 mM Tris-HCl, pH 8 at 30°C

656289

0.48

1-nitroethane

Neurospora crassa

Q01284

pH and temperature not specified in the publication

764000

-

1.1

1-nitrohexane

Neurospora crassa

Q01284

in 50 mM Tris-HCl, pH 8 at 30°C

656289

0.61

1-nitropentane

Neurospora crassa

Q01284

in 50 mM Tris-HCl, pH 8 at 30°C

656289

0.83

1-Nitropropane

2 entries

7.6

3-nitropropionate

2 entries

3.5

3-nitropropionic acid

Neurospora crassa

-

at pH 7.4 and 30°C

764031

5.6

butyl 1-nitronate

Neurospora crassa

Q01284

pH and temperature not specified in the publication

764000

-

5.6 - 112

butyl-1-nitronate

3 entries

2.5 - 300

ethyl nitronate

9 entries

1.02 - 129

ethylnitronate

4 entries

16

hexyl 1-nitronate

Neurospora crassa

Q01284

pH and temperature not specified in the publication

764000

-

16 - 130

hexyl-1-nitronate

2 entries

0.04 - 0.56

nitroethane

4 entries

0.001 - 42000

O2

6 entries

1.2

pentyl 1-nitronate

Neurospora crassa

Q01284

pH and temperature not specified in the publication

764000

-

1.2 - 121

pentyl-1-nitronate

3 entries

2000 - 14330

propionate 3-nitronate

3 entries

1300 - 11818.2

propionate-3-nitronate

4 entries

11.4

propyl 1-nitronate

Neurospora crassa

Q01284

pH and temperature not specified in the publication

764000

-

11.4 - 186.71

propyl-1-nitronate

3 entries

1.6 - 29

propyl-2-nitronate

2 entries

additional information

additional information

Neurospora crassa

-

investigation of the pH effects on the kcat/Km-values

672094

-

0.83

1-Nitropropane

Neurospora crassa

Q01284

in 50 mM Tris-HCl, pH 8 at 30°C

656289

0.83

1-Nitropropane

Neurospora crassa

Q01284

pH and temperature not specified in the publication

764000

7.6

3-nitropropionate

Neurospora crassa

-

pH 7.4, 30°C

726808

7.6

3-nitropropionate

Neurospora crassa

Q01284

pH and temperature not specified in the publication

764000

5.6

butyl-1-nitronate

Neurospora crassa

Q01284

in 50 mM Tris-HCl, pH 8 at 30°C

656289

33

butyl-1-nitronate

Pseudomonas aeruginosa

Q9HWH9

pH 7.5, 30°C, recombinant enzyme

745306

112

butyl-1-nitronate

Cyberlindnera mrakii

Q12723

-

684712

2.5

ethyl nitronate

Neurospora crassa

-

at pH 9.0 and 30°C

672094

16.5

ethyl nitronate

Neurospora crassa

Q01284

in 50 mM Tris-HCl, pH 8 at 30°C

656289

16.5

ethyl nitronate

Neurospora crassa

Q01284

pH and temperature not specified in the publication

764000

16.9

ethyl nitronate

Neurospora crassa

-

at pH 8.0 and 30°C

672094

25.4

ethyl nitronate

Neurospora crassa

-

at pH 6.0 and 30°C

672094

41

ethyl nitronate

Cyberlindnera saturnus

-

pH 11, 30°C

727018

46

ethyl nitronate

Cyberlindnera saturnus

-

pH 6, 30°C

727018

200

ethyl nitronate

Cyberlindnera saturnus

-

pH 8, 30°C

727018

300

ethyl nitronate

Cyberlindnera saturnus

-

pH 5.5, 30°C

727018

1.02

ethylnitronate

Neurospora crassa

-

30°C, pH 8, wild-type enzyme

685283

16.8

ethylnitronate

Neurospora crassa

-

30°C, pH 8, mutant enzyme H196N

685283

70

ethylnitronate

Pseudomonas aeruginosa

Q9HWH9

pH 7.5, 30°C, recombinant enzyme

745306

129

ethylnitronate

Cyberlindnera mrakii

Q12723

-

684712

16

hexyl-1-nitronate

Neurospora crassa

Q01284

in 50 mM Tris-HCl, pH 8 at 30°C

656289

130

hexyl-1-nitronate

Cyberlindnera mrakii

Q12723

-

684712

0.04

nitroethane

Neurospora crassa

-

at pH 6.0 and 30°C

672094

0.375

nitroethane

Neurospora crassa

-

at pH 9.0 and 30°C

672094

0.48

nitroethane

Neurospora crassa

Q01284

in 50 mM Tris-HCl, pH 8 at 30°C

656289

0.56

nitroethane

Neurospora crassa

-

at pH 8.0 and 30°C

672094

0.001

O2

Neurospora crassa

-

30°C, pH 8, wild-type enzyme

685283

0.0055

O2

Neurospora crassa

-

30°C, pH 8, mutant enzyme H196N

685283

11000

O2

Cyberlindnera saturnus

-

pH 5.5, 30°C

727018

15000

O2

Cyberlindnera saturnus

-

at pH 7.5 and 30°C

765734

33000

O2

Neurospora crassa

-

kcat_Km at least 33000 1/sec*mM with propionate 3-nitronate as cosubstrate, at pH 7.4 and 30°C

764031

42000

O2

Neurospora crassa

-

kcat_Km at least 42000 1/sec*mM, with 3-nitropropionic acid as cosubstrate, at pH 7.4 and 30°C

764031

1.2

pentyl-1-nitronate

Neurospora crassa

Q01284

in 50 mM Tris-HCl, pH 8 at 30°C

656289

91

pentyl-1-nitronate

Pseudomonas aeruginosa

Q9HWH9

pH 7.5, 30°C, recombinant enzyme

745306

121

pentyl-1-nitronate

Cyberlindnera mrakii

Q12723

-

684712

2000

propionate 3-nitronate

Neurospora crassa

-

at pH 7.4 and 30°C

764031

10000

propionate 3-nitronate

Cyberlindnera saturnus

-

at pH 7.5 and 30°C

765734

14330

propionate 3-nitronate

Cyberlindnera saturnus

-

pH 5.5, 30°C

727018

1300

propionate-3-nitronate

Neurospora crassa

-

pH 7.4, 30°C

726808

1300

propionate-3-nitronate

Neurospora crassa

Q01284

pH and temperature not specified in the publication

764000

2800

propionate-3-nitronate

Cyberlindnera saturnus

-

pH 7.4, 30°C

726808

11818.2

propionate-3-nitronate

Pseudomonas aeruginosa

Q9HWH9

pH 7.5, 30°C, recombinant enzyme

745306

11.4

propyl-1-nitronate

Neurospora crassa

Q01284

in 50 mM Tris-HCl, pH 8 at 30°C

656289

170

propyl-1-nitronate

Cyberlindnera mrakii

Q12723

-

684712

186.71

propyl-1-nitronate

Pseudomonas aeruginosa

Q9HWH9

pH 7.5, 30°C, recombinant enzyme

745306

1.6

propyl-2-nitronate

Cyberlindnera mrakii

Q12723

-

684712

29

propyl-2-nitronate

Pseudomonas aeruginosa

Q9HWH9

pH 7.5, 30°C, recombinant enzyme

745306