1.13.12.16: nitronate monooxygenase
This is an abbreviated version!
For detailed information about nitronate monooxygenase, go to the full flat file.
Word Map on EC 1.13.12.16
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1.13.12.16
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nitroalkanes
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flavin
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nitroethane
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neurospora
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crassa
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fmn
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hansenula
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mrakii
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flavosemiquinone
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1-nitropropane
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denitrification
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3-nitropropionic
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ansochromogenes
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fmn-dependent
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saturnus
- 1.13.12.16
- nitroalkanes
- flavin
- nitroethane
- neurospora
- crassa
- fmn
- hansenula
- mrakii
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flavosemiquinone
- 1-nitropropane
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denitrification
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3-nitropropionic
- ansochromogenes
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fmn-dependent
- saturnus
Reaction
Synonyms
2-nitropropane dioxygenase, EC 1.13.11.32, NAO, ncd2, nitroalkane oxidase, nitroalkane-oxidizing enzyme, nitronate monooxygenase, nitropropane dioxygenase, NMO, NMO-Nc, NMO-Ws, nmoA, Npd1, Npd2, Npd3, Npd4, Npd5, Npd6, oxidase, nitroalkane, oxygenase, 2-nitropropane di-, P3N monooxygenase, Pa-NMO, PA4202, protein PA4202, Rv1894c, Sa-NAO
ECTree
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Crystallization
Crystallization on EC 1.13.12.16 - nitronate monooxygenase
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hanging drop vapor diffusion method, using 21% (w/v) PEG 3350, 70 mM sodium citrate at pH 4.0, and 100 mM ammonium acetate
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hanging drop vapour diffusion method using 10% (w/v) polyethylene glycol monomethyl ether 5000
purified recombinant His-tagged enzyme, vapor diffusion hanging drop method at room temperature, 0.002 ml of 14 mg/ml protein in 50 mM Tris-Cl, pH 8.0, 100 mM NaCl, 20 mM 2-nitropropane, with 0.002 ml of reservoir solution containing 14% w/v PEG 5000 monomethylether and 0.1 M Na-HEPES, pH 7.0, and equilibration against 1 ml of reservoir solution, 7-10 days, X-ray diffraction structure determination and analysis at 1.44 A resolution, molecular replacement
crystal structure of NAO from Streptomyces ansochromogenes is determined. It consists of two domains, a TIM barrel domain bound to FMN and C-terminal domain with a novel folding pattern