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1.13.11.4: gentisate 1,2-dioxygenase

This is an abbreviated version!
For detailed information about gentisate 1,2-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.4

Reaction

2,5-Dihydroxybenzoate
+
O2
=
maleylpyruvate

Synonyms

2,5-dihydroxybenzoate dioxygenase, DsmD, EC 1.13.1.4, EC 1.99.2.4, eGDO, GDO, GDO-II, gdoA, GDOsp, GenH, gentisate dioxygenase, gentisate oxygenase, gentisate-1,2-dioxygenase, gentisic acid oxidase, gtdA-2, hbzE, Nagl1, Nagl2, Nagl3, NarI, oxygenase, gentisate 1,2-di-, P25X gentisate 1,2-dioxygenase, P35X gentisate 1,2-dioxygenase, salicylate 1,2-dioxygenase, SDO

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.4 gentisate 1,2-dioxygenase

Storage Stability

Storage Stability on EC 1.13.11.4 - gentisate 1,2-dioxygenase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 1 week, purified enzyme loses 51% of its initial activity
-20°C, addition of glycerol and beta-mercaptoethanol, 96 h, 25% of activity remains
-
-20°C, MOPS buffer with 0.1 mM Fe2+, 2 mM L-cysteine, and 10% (v/v) glycerol, 12 months, 3.5% residual activity
-
-20°C, MOPS buffer with 0.1 mM Fe2+, 2 mM L-cysteine, and 10% (v/v) glycerol, 12 months, 42.9% residual activity
-
-20°C, MOPS buffer with 0.1 mM Fe2+, 2 mM L-cysteine, and 10% (v/v) glycerol, 3 months, 42.5% residual activity
-
-20°C, MOPS buffer with 0.1 mM Fe2+, 2 mM L-cysteine, and 10% (v/v) glycerol, 3 months, 86.5% residual activity
-
-20°C, MOPS buffer with 0.1 mM Fe2+, 2 mM L-cysteine, and 10% (v/v) glycerol, 6 months, 23.1% residual activity
-
-20°C, MOPS buffer with 0.1 mM Fe2+, 2 mM L-cysteine, and 10% (v/v) glycerol, 6 months, 70.9% residual activity
-
-20°C, purified enzyme, 98 h, complete loss of activity
-70°C, 1 week, purified enzyme loses 30% of its initial activity
-70°C, 2 weeks, 70% loss of activity
-
-80°C, 2 weeks, purified enzyme loses 50% of its initial activity. Addition of 2.0 mM GSH (or cysteine), 0.1 mM Fe(NH4)2(SO4)2*6H2O, and 10% glycerol stabilizes the activity
-
-80°C, storage following rapid freezing in liquid N2, protein concentration above 10 mg/ml, about 15% loss of activity after 1 year
4°C, 48 h, complete loss of activity
-
4°C, 72 h, 90% loss of activity
-
4°C, MOPS buffer with no additives, 24 h, 22.5% residual activity
-
4°C, MOPS buffer with no additives, 24 h, 33.9% residual activity
-
4°C, MOPS buffer with no additives, 96 h, complete loss of activity
cell free extracts adsorbed on polyester filters, stable
-