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1.13.11.1: catechol 1,2-dioxygenase

This is an abbreviated version!
For detailed information about catechol 1,2-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.1

Reaction

catechol
+
O2
=
cis,cis-muconate

Synonyms

(chloro-)catechol 1,2-dioxygenase, 1,2 CTD, 1,2-CTD, 1,2-pyrocatechase, 3-chlorocatechol 1,2-dioxygenase, BC-C23O, C1,2-DO, C1,2O, C12, C12D, C12DO, C12O, cat12, CatA, catechase, catechol 1,2 dioxygenase, catechol 1,2-dioxygenase, catechol 1,2-dioxygenase type I, catechol 1,2-dioxygenase type II, catechol 1,2-oxygenase, catechol dioxygenase, catechol oxygenase, catechol-oxygen 1,2-oxidoreductase, catechol:oxygen 1,2-oxidoreductase, CD I, CD II, CD-I, CD-II, CD-III-1, CD-III-2, CDI1, CDI2, chlorocatechol 1,2-dioxygenase, clCA, extradiol dioxygenase MhpB, IsoB, putative catechol 2,3-dioxygenase, pyrocatechase, pyrocatechol 1,2-dioxygenase, TfdC

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.1 catechol 1,2-dioxygenase

Storage Stability

Storage Stability on EC 1.13.11.1 - catechol 1,2-dioxygenase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, pH 9.0, 5-10% loss of activity after several months
-
25°C, 11 days, complete inactivation for the immobilized protein
-
25°C, 4 days, residual activity 50% for the immobilized protein and 10% for the free form
-
25°C, 5 days, complete inactivation for the free enzyme
-
4°C, 10 days, 50% residual acitivity for the immobilized protein and only 10% residual activity of the free form protein
-
4°C, 100% activity after 1 month
-
4°C, 6 weeks, 39% loss of activity
Stutzerimonas stutzeri
4°C, EDTA buffer, pH 7.3
-
4°C, pH 7.6, 50 mM Tris/HCl, stable for two weeks
-
4°C, pH 8.5, stable over a period of several months
-
6°C, buffer containing 0.1 M NaCl, 50 days without decrease of activity
-
pyrocatechase I: 4°C, Tris-HCl buffer, pH 8.0, supplemented with 2-mercaptoethanol, stable over a period of 3 months
-
pyrocatechase II: at room temperature, Tris-HCl buffer, pH 8.0, supplemented with 2-mercaptoethanol, 10% loss of activity after 3 weeks, at -16°C for 24 hours 95% loss of activity
-