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1.13.11.1: catechol 1,2-dioxygenase

This is an abbreviated version!
For detailed information about catechol 1,2-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.1

Reaction

catechol
+
O2
=
cis,cis-muconate

Synonyms

(chloro-)catechol 1,2-dioxygenase, 1,2 CTD, 1,2-CTD, 1,2-pyrocatechase, 3-chlorocatechol 1,2-dioxygenase, BC-C23O, C1,2-DO, C1,2O, C12, C12D, C12DO, C12O, cat12, CatA, catechase, catechol 1,2 dioxygenase, catechol 1,2-dioxygenase, catechol 1,2-dioxygenase type I, catechol 1,2-dioxygenase type II, catechol 1,2-oxygenase, catechol dioxygenase, catechol oxygenase, catechol-oxygen 1,2-oxidoreductase, catechol:oxygen 1,2-oxidoreductase, CD I, CD II, CD-I, CD-II, CD-III-1, CD-III-2, CDI1, CDI2, chlorocatechol 1,2-dioxygenase, clCA, extradiol dioxygenase MhpB, IsoB, putative catechol 2,3-dioxygenase, pyrocatechase, pyrocatechol 1,2-dioxygenase, TfdC

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.1 catechol 1,2-dioxygenase

Specific Activity

Specific Activity on EC 1.13.11.1 - catechol 1,2-dioxygenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.01
-
whole cell induced by D-fructose, pH 7.4, 30°C
0.02
-
growth condition: LB medium added with 500mg/l catechol
0.041
-
substrate 3-chlorocatechol, crude enzyme extract
0.06
inductor phenol, cell-free extract from Stenotrophomonas maltophilia
0.12
inductor vanillic acid, cell-free extract from Stenotrophomonas maltophilia
0.121
-
3-chlorocatechol
0.137
-
substrate 4-chlorocatechol, crude enzyme extract
0.138
-
Intracellular enzyme activity of C12D in crude lysate
0.14
-
cells cultivated with benzoate
0.16
-
cells cultivated with phenol
0.172
-
4-chlorocatechol
0.19
-
whole cell induced by 2,4-dichlorophenoxyacetate, pH 7.4, 30°C
0.246
-
substrate catechol, crude enzyme extract
0.348
-
pyrocatechase II, at room temperature, instable to chromatography and dilution
0.41
-
whole cell induced by 2-chloro-4-methylphenoxyacetic acid, pH 7.4, 30°C
0.5
-
growth condition: catechol
0.64
-
catechol
0.87
-
crude enzyme
1.03
inductor catechol, cell-free extract from Stenotrophomonas maltophilia
1.14
-
growth condition: tryptone medium added with 500 mg/l catechol
1.2
pH not specified in the publication, 25°C, substrate 4-chlorocatechol
1.4
pH not specified in the publication, 25°C, substrate 3-methylcatechol
1.42
-
crude enzyme, at pH 7.0 and 30°C
11.97
inductor benzoic acid, cell-free extract from Stenotrophomonas maltophilia
12
-
purified isozyme 3
12.3
pH not specified in the publication, 25°C, substrate catechol
15
Stutzerimonas stutzeri
pH 8.5, temperature not specified in the publication
15.13
-
afer 10.6fold purification, at pH 7.0 and 30°C
15.3
pH not specified in the publication, 25°C, substrate catechol
17.9
-
purified isozyme 2
2.9
pH not specified in the publication, 25°C, substrate 4-chlorocatechol
22.4
-
purified isozyme 1
3.8
pH not specified in the publication, 25°C, substrate 3-methylcatechol
34
-
enzyme purified from L-malate grown cells
35
-
enzyme purified from aniline-grown cells
35.8
pH not specified in the publication, 25°C, substrate 4-methylcatechol
36
-
CD-III-2
37.65
-
after 319fold purification, at pH 7.2 and 45°C
4.4
pH not specified in the publication, 25°C, substrate 4-methylcatechol
41
-
CD-III-1
5.4
-
purified recombinant enzyme
672
purified enzyme, pH 7.4, 35°C
additional information