1.13.11.1: catechol 1,2-dioxygenase
This is an abbreviated version!
For detailed information about catechol 1,2-dioxygenase, go to the full flat file.
Reaction
+
=
Synonyms
(chloro-)catechol 1,2-dioxygenase, 1,2 CTD, 1,2-CTD, 1,2-pyrocatechase, 3-chlorocatechol 1,2-dioxygenase, BC-C23O, C1,2-DO, C1,2O, C12, C12D, C12DO, C12O, cat12, CatA, catechase, catechol 1,2 dioxygenase, catechol 1,2-dioxygenase, catechol 1,2-dioxygenase type I, catechol 1,2-dioxygenase type II, catechol 1,2-oxygenase, catechol dioxygenase, catechol oxygenase, catechol-oxygen 1,2-oxidoreductase, catechol:oxygen 1,2-oxidoreductase, CD I, CD II, CD-I, CD-II, CD-III-1, CD-III-2, CDI1, CDI2, chlorocatechol 1,2-dioxygenase, clCA, extradiol dioxygenase MhpB, IsoB, putative catechol 2,3-dioxygenase, pyrocatechase, pyrocatechol 1,2-dioxygenase, TfdC
ECTree
KM Value
KM Value on EC 1.13.11.1 - catechol 1,2-dioxygenase
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0.0093
1,2,3-benzenetriol
-
at pH 7.2 and 45°C
0.7
2,3-Dihydroxybenzoic acid
in 50 mM Tris/HCl, pH 7.2, 25°C
0.0007 - 0.002
3-chlorocatechol
0.0044
3-ethylcatechol
-
-
0.001 - 0.286
3-methylcatechol
0.0005
3-methylthiocatechol
-
-
0.02
4,5-difluorocatechol
-
-
0.0001 - 0.64
4-Chlorocatechol
0.0007 - 0.02
4-Fluorocatechol
0.0012 - 0.435
4-methylcatechol
0.097
Hydroxyquinol
in 50 mM Tris/HCl, pH 7.2, 25°C
0.31
protocatechuate
in 50 mM Tris/HCl, pH 7.2, 25°C
0.0045
pyrogallol
in 50 mM Tris/HCl, pH 7.2, 25°C
0.0005
tetrachlorocatechol
-
-
additional information
additional information
-
0.0007
3-chlorocatechol
in 50 mM Tris/HCl, pH 7.2, 25°C
0.002
3-chlorocatechol
-
-
0.001
3-methylcatechol
pH not specified in the publication, 25°C
0.0017
3-methylcatechol
-
-
0.00225
3-methylcatechol
wild-type enzyme
0.00225
3-methylcatechol
wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication
0.0032
3-methylcatechol
-
-
0.0037
3-methylcatechol
-
-
0.0048
3-methylcatechol
-
at pH 7.2 and 45°C
0.0056
3-methylcatechol
pH not specified in the publication, 25°C
0.00595
3-methylcatechol
mutant L69A
0.00595
3-methylcatechol
mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication
0.0065
3-methylcatechol
in 50 mM Tris/HCl, pH 7.2, 25°C
0.009
3-methylcatechol
-
-
0.286
3-methylcatechol
-
-
0.0001
4-Chlorocatechol
mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication
0.000101
4-Chlorocatechol
mutant L69A
0.0005
4-Chlorocatechol
-
-
0.001
4-Chlorocatechol
pH not specified in the publication, 25°C
0.0015
4-Chlorocatechol
in 50 mM Tris/HCl, pH 7.2, 25°C
0.00198
4-Chlorocatechol
wild-type enzyme
0.00198
4-Chlorocatechol
wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication
0.00242
4-Chlorocatechol
mutant L69G/A72G
0.0061
4-Chlorocatechol
mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication
0.00613
4-Chlorocatechol
mutant A72G
0.00654
4-Chlorocatechol
mutant A72S
0.0501
4-Chlorocatechol
mutant A72N
0.17
4-Chlorocatechol
-
-
0.2081
4-Chlorocatechol
mutant A72D
0.64
4-Chlorocatechol
-
at pH 7.2 and 45°C
0.0007
4-Fluorocatechol
-
-
0.02
4-Fluorocatechol
-
-
0.0012
4-methylcatechol
-
-
0.0017
4-methylcatechol
pH not specified in the publication, 25°C
0.00284
4-methylcatechol
mutant L69G/A72G
0.0035
4-methylcatechol
-
at pH 7.2 and 45°C
0.004
4-methylcatechol
in 50 mM Tris/HCl, pH 7.2, 25°C
0.0068
4-methylcatechol
-
-
0.0099
4-methylcatechol
pH not specified in the publication, 25°C
0.01
4-methylcatechol
mutant A72G
0.01
4-methylcatechol
mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication
0.0128
4-methylcatechol
mutant L69A
0.0128
4-methylcatechol
mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication
0.013
4-methylcatechol
-
-
0.014
4-methylcatechol
-
-
0.018
4-methylcatechol
-
-
0.0215
4-methylcatechol
-
25°C
0.053
4-methylcatechol
wild-type enzyme
0.053
4-methylcatechol
wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication
0.09
4-methylcatechol
-
-
0.0936
4-methylcatechol
mutant A72D
0.213
4-methylcatechol
mutant A72S
0.435
4-methylcatechol
mutant A72N
0.00025
catechol
-
-
0.0008
catechol
pH not specified in the publication, 25°C
0.0015
catechol
-
immobilized enzyme
0.00185
catechol
-
at pH 7.2 and 45°C
0.0019
catechol
-
isoenzymes 1 and 3
0.002
catechol
-
isoenzyme 2
0.002
catechol
-
free protein
0.00204
catechol
wild-type enzyme
0.00204
catechol
wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication
0.00235
catechol
mutant A72N
0.003
catechol
in 50 mM Tris/HCl, pH 7.2, 25°C
0.00576
catechol
mutant L69A
0.00576
catechol
mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication
0.0075
catechol
-
free enzyme
0.0075
catechol
pH not specified in the publication, 25°C
0.0092
catechol
-
at pH 7.5 and 30°C
0.0093
catechol
mutant A72G
0.0093
catechol
mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication
0.01
catechol
mutant A72S
0.0128
catechol
pH 8.0, 40°C
0.0132
catechol
Stutzerimonas stutzeri
pH 8.5, temperature not specified in the publication
0.0166
catechol
-
immobilized protein
0.01753
catechol
-
at pH 7.0 and 30°C
0.03997
catechol
pH 8.0, 35°C
0.0428
catechol
mutant L69G/A72G
0.0691
catechol
mutant A72D
0.06
oxygen
-
-
0.096
oxygen
-
isoenzymes 1 and 2
0.098
oxygen
-
isoenzyme 3
additional information
additional information
-
-
-
additional information
additional information
Michaelis-Menten kinetics, overview
-
additional information
additional information
-
Michaelis-Menten kinetics, overview
-