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1.12.1.3: hydrogen dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about hydrogen dehydrogenase (NADP+), go to the full flat file.

Word Map on EC 1.12.1.3

Reaction

H2
+
NADP+
=
H+
 +
NADPH

Synonyms

hydrogenase [ambiguous], NADP-dependent hydrogenase, NADP-linked hydrogenase, NADP-reducing hydrogenase, NADPH-dependent hydrogenase I, NADPH-dependent [NiFe]-hydrogenase, NiFe-hydrogenase, SHI, soluble hydrogenase I, [NiFe]-hydrogenase, [NiFe]-hydrogenase I

ECTree

     1 Oxidoreductases
         1.12 Acting on hydrogen as donor
             1.12.1 With NAD+ or NADP+ as acceptor
                1.12.1.3 hydrogen dehydrogenase (NADP+)

Purification

Purification on EC 1.12.1.3 - hydrogen dehydrogenase (NADP+)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
an active, purified 12-His tagged recombinant SHI (rSHI) is obtained by one-step affinity adsorption on nickel-charged resin
-
C-terminal domain of subunit HndA and N-terminal domain of subunit HndD recombinantly expressed in Escherichia coli
Solidesulfovibrio fructosivorans
-
HndA subunit
Solidesulfovibrio fructosivorans
-
one-step purification of the recombinant enzyme including a polyhistidine affinity tag results in a 50fold increase in yield compared to the four-step purification procedure for the native enzyme
single affinity chromatography, 4°C, aerobic conditions
-
strategies for reliable and improved large-scale production of Pyrococcus furiosus with integrated purification of hydrogenase I
-
truncated version of HndA subunit
Solidesulfovibrio fructosivorans
-