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1.12.1.2: hydrogen dehydrogenase

This is an abbreviated version!
For detailed information about hydrogen dehydrogenase, go to the full flat file.

Word Map on EC 1.12.1.2

Reaction

H2
+
NAD+
=
H+
 +
NADH

Synonyms

bidirectional hydrogenase, bidirectional Ni-Fe hydrogenase, bidirectional NiFe-hydrogenase, bidirectional [NiFe] hydrogenase, dehydrogenase, hydrogen, FeHyd, H2:NAD+ oxidoreductase, H2ase, Hox, Hox hydrogenase, Hox type hydrogenase, HoxE, HoxEFUYH, HoxF, HoxFUYHI2, HoxH, HoxS, HoxU, HoxY, hydrogen-evolving enzyme, hydrogenase, Hyn, iron-hydrogenase, NAD+-reducing hydrogenase, NAD+-reducing NiFe hydrogenase, NAD+-reducing [NiFe]-hydrogenase, NAD-dependent hydrogenase, NAD-reducing hydrogenase, NADH-dependent Fe-only hydrogenase, NiFe hydrogenase, NiFe-type reversible hydrogenase, SH, soluble hydrogenase, soluble [NiFe]-hydrogenase, [FeFe] hydrogenase, [FeFe]-hydrogenase, [NiFe] H2ase, [NiFe] hydrogenase, [NiFe]-hydrogenase

ECTree

     1 Oxidoreductases
         1.12 Acting on hydrogen as donor
             1.12.1 With NAD+ or NADP+ as acceptor
                1.12.1.2 hydrogen dehydrogenase

Specific Activity

Specific Activity on EC 1.12.1.2 - hydrogen dehydrogenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0006
0.0017
-
soluble periplasmic fraction, hydrogen producing activity with electron acceptor oxidized methyl viologen
0.0018
cell culture in nitrogen-limited medium, anaerobic conditions, H2 production using reduced methyl viologen as electron donor
0.0029
-
soluble periplasmic fraction, hydrogen producing activity with electron donor NADH
0.006
-
soluble periplasmic fraction, hydrogen producing activity with electron donor reduced benzyl viologen
0.0095
-
soluble periplasmic fraction, hydrogen producing activity with electron acceptor oxidized benzyl viologen
0.0113
-
soluble periplasmic fraction, hydrogen producing activity with electron donor reduced methyl viologen
0.0175
-
soluble periplasmic fraction, hydrogen producing activity with electron acceptor oxidized methylene blue
0.0255
-
soluble periplasmic fraction, hydrogen producing activity with electron acceptor NAD+
0.035
-
H2 producing activity, strain R43 grown without nitrogen and nickel, from host plant Compotonia cunninghamiana, with reduced methyl viologen as electron donor
0.076
-
crude extract, pH and temperature not specified in the publication
0.11
-
H2 producing activity, strain UGL011101 grown without nitrogen and nickel, from host plant Alnus incana, with reduced methyl viologen as electron donor
0.3
-
H2 producing activity, strain HFPCcI3 grown without nitrogen and nickel, from host plant Compotonia cunninghamiana, with reduced methyl viologen as electron donor
1.7
-
recombinant enzyme expressed in Ralstonia eutropha
10
-
purified enzyme, H2 production
100
-
electron acceptor benzyl viologen
102.1
-
reaction with H2 and ferrocyanide, purified HoxI deletion mutant enzyme
108.8
-
reaction with H2 and ferrocyanide, purified wild-type enzyme complex including HoxI
125 - 175
-
activity of different batches with NADH and ferricyanide
1263
-
activity of different batches with benzyl viologen and H2
1300
-
purified enzyme, with substrates oxidized benzyl viologen and NADH
15
native purified enzyme complex from strain PCC 6301, hydrogen-forming activity, cofactor reduced methyl viologen
17 - 84
-
activity of different batches with NAD+ and H2
1700
-
purified enzyme, both reaction directions with substrates oxidized/reduced methyl viologen and H2/H+
2030
-
purified hydrogenase, at 30°C
230
-
recombinant protein, pH 8.0, temperature not specified in the publication
25.2
-
reaction with H2 and benzyl viologen, purified HoxI deletion mutant enzyme
30 - 100
-
purified enzyme, forward reaction under aerobic conditions with NAD+
34
-
electron acceptor NAD+
35.1
-
reaction with H2 and benzyl viologen, purified wild-type enzyme complex including HoxI
36.5
Hydrogenomonas sp.
-
-
38
-
strain H16, electron acceptor NAD+
39
-
reaction with H2 and NAD+, purified HoxI deletion mutant enzyme
4.91
-
H2 producing activity, strain UGL140102 grown without nitrogen and nickel, from host plant Hippophae rhamnoides, with reduced methyl viologen as electron donor
41.9
-
reaction with H2 and NAD+, purified wild-type enzyme complex including HoxI
430
-
pH 6.0, 40°C, measured by hydrogen uptake with methyl viologen as electron acceptor
5
-
purified enzyme, H2 consumption
5.6
-
strain Cd2/01, electron acceptor NAD+
61.6
-
reaction with NADH and ferrocyanide, purified HoxI deletion mutant enzyme
64.9
-
reaction with NADH and ferrocyanide, purified wild-type enzyme complex including HoxI
67
native purified enzyme complex from strain PCC 6803, hydrogen-forming activity, cofactor reduced methyl viologen
7.7
-
electron acceptor NAD+
72
-
electron acceptor NAD+
85
-
electron acceptor NAD+
87.78
-
after 1155old purification, pH and temperature not specified in the publication
additional information