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1.12.1.2: hydrogen dehydrogenase

This is an abbreviated version!
For detailed information about hydrogen dehydrogenase, go to the full flat file.

Word Map on EC 1.12.1.2

Reaction

H2
+
NAD+
=
H+
 +
NADH

Synonyms

bidirectional hydrogenase, bidirectional Ni-Fe hydrogenase, bidirectional NiFe-hydrogenase, bidirectional [NiFe] hydrogenase, dehydrogenase, hydrogen, FeHyd, H2:NAD+ oxidoreductase, H2ase, Hox, Hox hydrogenase, Hox type hydrogenase, HoxE, HoxEFUYH, HoxF, HoxFUYHI2, HoxH, HoxS, HoxU, HoxY, hydrogen-evolving enzyme, hydrogenase, Hyn, iron-hydrogenase, NAD+-reducing hydrogenase, NAD+-reducing NiFe hydrogenase, NAD+-reducing [NiFe]-hydrogenase, NAD-dependent hydrogenase, NAD-reducing hydrogenase, NADH-dependent Fe-only hydrogenase, NiFe hydrogenase, NiFe-type reversible hydrogenase, SH, soluble hydrogenase, soluble [NiFe]-hydrogenase, [FeFe] hydrogenase, [FeFe]-hydrogenase, [NiFe] H2ase, [NiFe] hydrogenase, [NiFe]-hydrogenase

ECTree

     1 Oxidoreductases
         1.12 Acting on hydrogen as donor
             1.12.1 With NAD+ or NADP+ as acceptor
                1.12.1.2 hydrogen dehydrogenase

Purification

Purification on EC 1.12.1.2 - hydrogen dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
20fold by several chromatographic steps
-
hexameric enzyme form
-
improved purification procedure
-
partially, preparation of periplasmic fraction
-
protamine sulfate, ammonium sulfate, DEAE-cellulose, Sephadex G-200
-
recombinant protein. The as-isolated module HoxHY is initially catalytically inactive, but after reductive activation at low potentials, exhibits both H2 oxidation and H+ reduction
recombinant Strep-tagged HoxI-deletion mutant tetramer from Escherichia coli by 2 different procedures
-
sp. H16, cetavlon, ammonium sulfate, DEAE-cellulose, Sephadex G-200, hydroxyapatite
-
Strep-Tactin-Sepharose column chromatography
-
under anaerobic conditions, pentameric bidirectional hydrogenase complex HoxEFUYH to near homogeneity 647fold from strain PCC 6803 and 1290fold from strain PCC 6301, recombinant His-tagged HoxE from Escherichia coli