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1.12.1.2: hydrogen dehydrogenase

This is an abbreviated version!
For detailed information about hydrogen dehydrogenase, go to the full flat file.

Word Map on EC 1.12.1.2

Reaction

H2
+
NAD+
=
H+
 +
NADH

Synonyms

bidirectional hydrogenase, bidirectional Ni-Fe hydrogenase, bidirectional NiFe-hydrogenase, bidirectional [NiFe] hydrogenase, dehydrogenase, hydrogen, FeHyd, H2:NAD+ oxidoreductase, H2ase, Hox, Hox hydrogenase, Hox type hydrogenase, HoxE, HoxEFUYH, HoxF, HoxFUYHI2, HoxH, HoxS, HoxU, HoxY, hydrogen-evolving enzyme, hydrogenase, Hyn, iron-hydrogenase, NAD+-reducing hydrogenase, NAD+-reducing NiFe hydrogenase, NAD+-reducing [NiFe]-hydrogenase, NAD-dependent hydrogenase, NAD-reducing hydrogenase, NADH-dependent Fe-only hydrogenase, NiFe hydrogenase, NiFe-type reversible hydrogenase, SH, soluble hydrogenase, soluble [NiFe]-hydrogenase, [FeFe] hydrogenase, [FeFe]-hydrogenase, [NiFe] H2ase, [NiFe] hydrogenase, [NiFe]-hydrogenase

ECTree

     1 Oxidoreductases
         1.12 Acting on hydrogen as donor
             1.12.1 With NAD+ or NADP+ as acceptor
                1.12.1.2 hydrogen dehydrogenase

Engineering

Engineering on EC 1.12.1.2 - hydrogen dehydrogenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C461A
-
site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, inactive
D456N
-
site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, nearly inactive
D456S
-
site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, nearly inactive
E14Q
-
site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, inactive
E14V
-
site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, nearly inactive
G15A
-
site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, nearly inactive
H396L
-
site-directed mutagenesis, catalytic subunit HoxH allele, highly reduced activity compared to the wild-type strain
H396Q
-
site-directed mutagenesis, catalytic subunit HoxH allele, about 3.5fold reduced activity compared to the wild-type strain
H69Q
-
site-directed mutagenesis, catalytic subunit HoxH allele, about 5fold reduced activity compared to the wild-type strain
H69Q/P390A
-
site-directed mutagenesis, catalytic subunit HoxH alleles, reduced activity compared to the wild-type strain
I64A
-
site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, highly reduced activity compared to the wild-type strain
L118A
-
site-directed mutagenesis, catalytic subunit HoxH allele, reduced activity compared to the wild-type strain
L118F
-
site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow at O2 concentrations above 5%, about 4fold reduced activity compared to the wild-type strain
L118I
-
site-directed mutagenesis, catalytic subunit HoxH allele, reduced activity compared to the wild-type strain
L394A
-
site-directed mutagenesis, catalytic subunit HoxH allele, about 5fold reduced activity compared to the wild-type strain
L394N
-
site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, nearly inactive
P390A
-
site-directed mutagenesis, catalytic subunit HoxH allele, about 5fold reduced activity compared to the wild-type strain
R12L
-
site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, highly reduced activity compared to the wild-type strain
R12Q
-
site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, highly reduced activity compared to the wild-type strain
R391L
-
site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, inactive
R391Q
-
site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, inactive
R60Q
-
site-directed mutagenesis, catalytic subunit HoxH allele, highly reduced activity compared to the wild-type strain
S68V
-
site-directed mutagenesis, catalytic subunit HoxH allele, highly reduced activity compared to the wild-type strain
T415S
-
site-directed mutagenesis, catalytic subunit HoxH allele, about 5fold reduced activity compared to the wild-type strain
T415V
-
site-directed mutagenesis, catalytic subunit HoxH allele, highly reduced activity compared to the wild-type strain
T415V/N415H
-
site-directed mutagenesis, catalytic subunit HoxH alleles, O2-sensitive growth, highly reduced activity compared to the wild-type strain
I64M
-
the mutation alters gas diffusion kinetics and improves O2 tolerance
I64M/L107M
-
the mutant shows strongly reduced activity compared to the wild type enzyme
I64M/L107M/L112M
-
the mutant shows strongly reduced activity compared to the wild type enzyme
I64M/L112M
-
the mutant shows strongly reduced activity compared to the wild type enzyme
L107F
-
the mutant shows reduced activity compared to the wild type enzyme
L107M/L112M
-
the mutant shows reduced activity compared to the wild type enzyme
L112F
-
the mutant shows strongly reduced activity compared to the wild type enzyme
L112M
-
the mutant shows slightly reduced activity compared to the wild type enzyme
additional information