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1.11.1.16: versatile peroxidase

This is an abbreviated version!
For detailed information about versatile peroxidase, go to the full flat file.

Word Map on EC 1.11.1.16

Reaction

1-(4-hydroxy-3-methoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol
+
H2O2
=
4-hydroxy-3-methoxybenzaldehyde
 +
2-methoxyphenol
 +
glycolaldehyde
 +
H2O

Synonyms

B-type dye-decolorizing peroxidase, bacterial lignin peroxidase, DypB, manganese peroxidase 4, Mb peroxidase, metMb peroxidase, Mnp4, More, myoglobin, R1B4, versatile peroxidase, versatile peroxidase MnP2, versatile peroxidase VPL2 precursor, VP1, Vpl2, VPS1

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.16 versatile peroxidase

Cloned

Cloned on EC 1.11.1.16 - versatile peroxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a recombinant mnp2 construct under the control of Pleurotus ostreatus sdi1 expression signals is introduced into the wild-type Pleurotus ostreatus strain by cotransformation with a carboxin-resistant marker plasmid. Recombinant Pleurotus ostreatus strains with elevated manganese peroxidase (MnP) productivity are successfully isolated. The productivity of the recombinant MnP2 in the present system is not high enough to meet the requirements for industrial applications
-
all attempts to express a functional versatile peroxidase as soluble protein in Escherichia coli fail, recombinant enzyme expression and secretion from Saccharomyces cerevisiae, overexpression of enzyme mutant E37K/V160A/T184M/Q202L in Pichia pastoris
design of a synthetic gene encoding the N246Avariant of DypB from Rhodococcus jostii strain RHA1 (Rh_DypB) is designed by back translation of the protein sequence, recombinant overexpression of His-tagged N246A mutant enzyme in Escherichia coli strain BL21(DE3), the enzyme is fully produced as folded holoenzyme, thus without the need for a further reconstitution step
DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant enzyme expression in Nicotiana tabacum cv. Petite Havana via an Agrobacterium tumefaciens strain LBA4404 transformation procedure, expression under control of the CaMV 35S promoter. Selection of the VP lines VP22, VP24, and VP27 with higher MnP (EC 1.11.1.13) activities
expressed in Escherichia coli Top10 cells
-
expressed in Escherichia coli W3110 cells
expressed in Hansenula polymorpha strain RB11
expressed in Saccharomyces cerevisiae
expression in Aspergillus nidulans and Aspergillus niger
-
expression in Escherichia coli
expression in Escherichia coli fused to a thioredoxin-hexahistidine tag. Activity of the enzyme increases after removing the tag
expression in Escherichia coli W3110
expression in Saccharomyces cerevisiae
gene vp1, DNA and amino acid sequence determination and analysis, recombinant overexpression of His-tagged enzyme VP1 in inclusion body in Escherichia coli strain BL21(DE3), most derivatives of G-type lignin increase slightly by the recombinant enzyme rVP1 treatment compared with the control. The ratio of guaiacyl-type to syringyl-type derivatives (G/S) after rVP1 treatment is 5.4times higher than that of the control
-
generation of an enzyme saturation mutagenesis library by site-directed muztagenesis, recombinant expression of enzyme mutants in Saccharomyces cerevisiae strain EBY100, DNA and amino acid sequence determination and analysis.The recombinant enzyme shows microheterogeneity due to hyperglycosylation in Saccharomyces cerevisiae
recombinant MnP2 is exclusively expressed and no endogenous MnP isozymes are secreted by the recombinant Pleurotus oseatus srain TM2-18
-