Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.1.99.18: cellobiose dehydrogenase (acceptor)

This is an abbreviated version!
For detailed information about cellobiose dehydrogenase (acceptor), go to the full flat file.

Word Map on EC 1.1.99.18

Reaction

cellobiose
+
acceptor
=
cellobiono-1,5-lactone
 +
reduced acceptor

Synonyms

cbdA, CBO, CBOR, Cdh, CDH IIA, CDH IIB, cdh-1, CDH1, Cdh2, CDHIIA, cellobiose (acceptor) 1-oxidoreductase, cellobiose dehydrogenase, cellobiose dehydrogenase IIA, cellobiose oxidase, cellobiose oxidoreductase, cellobiose [acceptor] 1-oxidoreductase, Cellobiose-quinone oxidoreductase, cellobiose:(acceptor) 1-oxidoreductase, cellobiose:quinone oxidoreductase, cellobiose:[acceptor] 1-oxidoreductase, DCHsr, dehydrogenase, cellobiose, EC 1.1.3.25, EC 1.1.5.1, MtCDH, oxidase, cellobiose, Thite_59724, TpCDH, TvCDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.99 With unknown physiological acceptors
                1.1.99.18 cellobiose dehydrogenase (acceptor)

Cloned

Cloned on EC 1.1.99.18 - cellobiose dehydrogenase (acceptor)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Aspergillus niger
expressed in Aspergillus oryzae Jal355 cells
-
expressed in Escherichia coli
-
expressed in Escherichia coli Pichia pastoris, Aspergillus niger, and Trichoderma reesei
Thermothelomyces fergusii
-
expressed in Neurospora crassa strain 2489
-
expressed in Pichia pastoris
Thermothelomyces fergusii
-
expressed in Pichia pastoris strain GS115 and Trichoderma reesei strain QM9414
expressed in Pichia pastoris strain KM71
expressed in Pichia pastoris strain X-33
-
expressed in Pichia pastoris strain X33
-
expressed in Pichia pastoris X-33
-
expressed in Pichia pastoris X-33 cells
Thermothelomyces myriococcoides
-
expressed in Saccharomyces cerevisiae strain BJ5465
Thermothelomyces myriococcoides
expression in Aspergillus oryzae
expression in Escherichia coli
-
expression in Pichia pastoris
Thermothelomyces myriococcoides
-
expression in Pichia pastoris under the control of the AOX1 methanol promoter, without affecting its kinetic characteristics
expression in Pichia pastoris X-33 cells
Thermothelomyces fergusii
expression in Pichia pastoris, enzyme retains catalytic and cellulose-binding properties of the wild-type enzyme
-
expression in Trichoderma reesei. Heterologous production of the enzyme (PcCDH) is faster and the yield higher than secretion by Phanerochaete chrysosporium. It does not need a cellulose-based medium that impedes efficient production and purification of the enzyme (PcCDH) by binding to the polysaccharide. The obtained high uniformity of Phanerochaete chrysosporium CDHTr glycoforms is very useful to investigate electron transfer characteristics in biosensors and biofuel cells, which are depending on the spatial restrictions inflicted by high-mannose N-glycan trees. The determined catalytic and electrochemical properties of PcCDHTr are very similar to those of PcCDH and the FAD cofactor occupancy is good, which advocates Trichoderma reesei as expression host for engineered PcCDH for biosensors and biofuel cells
recombinantly produced in Pichia pastoris X-33 cells
Thermothelomyces myriococcoides
-
transformation of Ura- strain Ura11 with linearized pM65H
wild type and mutant cellobiose dehydrogenase genes are cloned for heterologously expression in yeast Pichia pastoris