1.1.5.8: quinate/shikimate dehydrogenase (quinone)
This is an abbreviated version!
For detailed information about quinate/shikimate dehydrogenase (quinone), go to the full flat file.
Word Map on EC 1.1.5.8
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1.1.5.8
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quinoproteins
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gluconobacter
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oxydans
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calcoaceticus
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3-dehydroquinate
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acinetobacter
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d-glucose
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3-dehydroshikimate
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quinohemoprotein
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methylobacterium
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holo-forms
- 1.1.5.8
-
quinoproteins
- gluconobacter
- oxydans
- calcoaceticus
- 3-dehydroquinate
- acinetobacter
- d-glucose
- 3-dehydroshikimate
-
quinohemoprotein
-
methylobacterium
-
holo-forms
Reaction
Synonyms
dehydrogenase, quinate (pyrroloquinoline-quinone), EC 1.1.99.25, NAD(P)+-independent quinate dehydrogenase, NAD(P)-independent quinate dehydrogenase, PQQ-dependent dehydrogenase, PQQ-QDH, pyrroloquinoline quinone-dependent dehydrogenase, QDH, quiA, quinate dehydrogenase, quinate dehydrogenase (quinone), quinate:pyrroloquinoline-quinone 5-oxidoreductase, quinoprotein quinate dehydrogenase
ECTree
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Purification
Purification on EC 1.1.5.8 - quinate/shikimate dehydrogenase (quinone)
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recombinant enzyme from cell membranes by dilution in 10 mM Tris-HCl, pH 7.5, 1 mM CaCl2, and 1% w/v DDM at the final protein concentration of 10 mg/ml followed by incubation for 30 min at 4°C with gentle mixing and ultracentrifugation at 100000 × g for 30 min at 4°C. The resultant supernatant containing the solubilized membranes is resuspended with the same volume of 10 mM Tris-HCl, pH 7.5, and 1 mM CaCl2. The enzyme is solublized from membranes and purified by anion exchange chromatography, dialysis, and hydroxyapatite chromatography