1.1.3.48: 3-deoxy-alpha-D-manno-octulosonate 8-oxidase

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For detailed information about 3-deoxy-alpha-D-manno-octulosonate 8-oxidase, go to the full flat file.

Reaction

Synonyms

kdnB

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.3 With oxygen as acceptor

                1.1.3.48 3-deoxy-alpha-D-manno-octulosonate 8-oxidase

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Results	in table
19	AA Sequence
3	Cloned(Commentary)
3	Cofactor
1	Crystallization (Commentary)
12	General Information
3	Inhibitors
6	Metals/Ions
6	Natural Substrates/ Products (Substrates)
16	Organism
3	Pathway
1	PDB ID
1	pH Optimum
2	Protein Variants
2	Purification (Commentary)
1	Reaction
4	Reference
12	Substrates and Products (Substrate)
7	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]

Cloned

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Cloned on EC 1.1.3.48 - 3-deoxy-alpha-D-manno-octulosonate 8-oxidase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expressed in Escherichia coli Rosetta2-(DE3) cells

Shewanella oneidensis

Q8EEB0

737728

expression in Escherichia coli

Shewanella oneidensis

Q8EEB0

734255

gene kdnB, encoded in the Kdo8N biosynthetic cluster, DNA and amino acid sequence determination and analysis, subcloning and expression in Escherichia coli strains EC100D and WBB06 at 25°C, recombinant expression of the gene cluster for 8-amino-3,8-dideoxy-D-manno-octulosonic acid biosynthesis from Shewanella oneidensis in Escherichia coli results in lipid A containing 8-amino-3,8-dideoxy-D-manno-octulosonic acid, and in vitro assays confirm the enzymatic functionality converting 3-deoxy-D-manno-octulosonic acid to 8-amino-3,8-dideoxy-D-manno-octulosonic acid, with incorporation into the Kdo8N-lipid A domain of LPS by a metal-dependent oxidase followed by a glutamate-dependent aminotransferase, recombinant expression of His-tagged enzyme in Escherichia coi strain C41

Shewanella oneidensis

Q8EEB0

734255