1.1.3.47: 5-(hydroxymethyl)furfural oxidase

This is an abbreviated version!
For detailed information about 5-(hydroxymethyl)furfural oxidase, go to the full flat file.

Word Map on EC 1.1.3.47

1.1.3.47

synthesis

2,5-furandicarboxylic

biocatalyst

bio-based

biocatalytic

oxidases

putida

methylovorus

basilensis

fed-batch

petroleum-based

terephthalate

cupriavidus

flavin

ornithinolytica

biomass-derived

raoultella

eugenol

enantioselective

polyesters

cosolvents

one-pot

fusing

biotechnology

biofuel production

Reaction

Synonyms

5-hydroxymethylfurfural oxidase, AAO, AOX1, GaoB, Glox2, Glox3, GLRG_02805, HFMO, HMF oxidase, HMF/furfural oxidoreductase, HmfH, HMFO, MPQ_0130

ECTree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.1.3 With oxygen as acceptor

1.1.3.47 5-(hydroxymethyl)furfural oxidase

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Results	in table
70	AA Sequence
17	Application
11	Cloned(Commentary)
8	Cofactor
2	Crystallization (Commentary)
4	Expression
11	General Information
2	Inhibitors
74	kcat/KM [mM/s]
69	KM Value [mM]
5	Localization
1	Metals/Ions
1	Molecular Weight [Da]
15	Natural Substrates/ Products (Substrates)
3	Organic Solvent Stability
32	Organism
2	Pathway
2	PDB ID
8	pH Optimum
6	pH Range
3	pI Value
2	Posttranslational Modification
32	Protein Variants
5	Purification (Commentary)
6	Reaction
32	Reference
3	Specific Activity [micromol/min/mg]
1	Storage Stability
104	Substrates and Products (Substrate)
9	Subunits
26	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
1	Temperature Range [°C]
6	Temperature Stability [°C]
71	Turnover Number [1/s]

Natural Substrates Products

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Natural Substrates Products on EC 1.1.3.47 - 5-(hydroxymethyl)furfural oxidase

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2,5-diformylfuran + 2 O2

2,5-furandicarboxylic acid + H2O2

3 entries

5-(dihydroxymethyl)furan-2-carbaldehyde + O2

5-formylfuran-2-carboxylate + H2O2

Methylovorus sp. MP688

E4QP00

739768

5-(dihydroxymethyl)furan-2-carboxylate + O2

furan-2,5-dicarboxylate + H2O2

Methylovorus sp. MP688

E4QP00

739768

5-(hydroxymethyl)furfural + 3 O2 + 2 H2O

furan-2,5-dicarboxylate + 3 H2O2

3 entries

5-(hydroxymethyl)furfural + O2

furan-2,5-dicarbaldehyde + H2O2

Methylovorus sp. MP688

E4QP00

739768

5-formylfuran-2-carboxylate + H2O

5-(dihydroxymethyl)furan-2-carboxylate

Methylovorus sp. MP688

E4QP00

739768

5-hydroxymethylfurfural + O2

2,5-diformylfuran + H2O2

3 entries

furan-2,5-dicarbaldehyde + H2O

5-(dihydroxymethyl)furan-2-carbaldehyde

Methylovorus sp. MP688

E4QP00

739768

additional information

Pleurotus eryngii

O94219

the ability of fungal aryl-alcohol oxidase (AAO) to oxidize 5-hydroxymethylfurfural (HMF) results in almost complete conversion into 2,5-formylfurancarboxylic acid (FFCA) in a few hours. The reaction starts with alcohol oxidation, yielding 2,5-diformylfuran (DFF), which is rapidly converted into FFCA by carbonyl oxidation, most probably without leaving the enzyme active site. AAO is combined with an unspecific peroxygenase, UPO, EC 1.11.2.1, from Agrocybe aegerita for full oxidative conversion of 5-hydroxymethylfurfural in an enzymatic cascade. This peroxygenase belongs to the recently described superfamily of hemethiolate peroxidases, and is capable of incorporating peroxide-borne oxygen into diverse substrate molecules. In contrast to AAO, the UPO reaction starts with oxidation of the HMF carbonyl group, yielding 2,5-hydroxymethylfurancarboxylic, which is converted into 2,5-formylfurancarboxylic acid and some 2,5-furandicarboxylic acid

742493