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1.1.3.4: glucose oxidase

This is an abbreviated version!
For detailed information about glucose oxidase, go to the full flat file.

Word Map on EC 1.1.3.4

Reaction

beta-D-glucose
+
O2
=
D-glucono-1,5-lactone
 +
H2O2

Synonyms

AldO, beta-D-glucose oxidase, beta-D-glucose oxygen-1-oxidoreductase, beta-D-glucose/oxygen 1-oxidoreductase, beta-D-glucose: oxygen 1-oxidoreductase, beta-D-glucose:O2 1-oxidoreductase, beta-D-glucose:O2-1-oxidoreductase, beta-D-glucose:oxygen 1-oxido-reductase, beta-D-glucose:oxygen 1-oxidoreductase, beta-D-glucose:oxygen oxidoreductase, beta-D-glucose:oxygen-1-oxidoreductase, beta-D-glucose:quinone oxidoreductase, CngoxA, corylophyline, D-glucose oxidase, D-glucose-1-oxidase, deoxin-1, glucose aerodehydrogenase, glucose oxyhydrase, glucose-1-oxidase, GO-2, GOD, GOX, GOxP5, microcid, More, notatin, oxidase, glucose, pen-GOx, penatin, yGOXpenag

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.4 glucose oxidase

Purification

Purification on EC 1.1.3.4 - glucose oxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
all reconstituted variants are purified to homogeneity by mild acidification and ion-exchange chromatography
ammonium sulfate precipitation
ammonium sulfate precipitation and anion exchange column chromatography
-
ammonium sulfate precipitation, DEAE cellulose column chromatography, and Sephadex G-100 gel filtration
-
ammonium sulfate precipitation, ion exchange chromatography, and gel filtration
-
from a partially purified sample, using column chromatography on DEAE-cellulose
-
from BTL wild-type strain using ammonium sulfate precipitation, column chromatography on Q-Sepharose at pH 6 and pH 4.5 and gel filtration on SW-300
-
from commercial preparation
from commercial preparation using DEAE-cellulose chromatography and Sephadex G-200 gel filtration
-
G-25 column chromatography
-
HiTrap phenyl HP column chromatography and HiTrap Q FF column chromatography
-
isolation and purification of the extracellular enzyme by precipitation with Mg(OH)2, Zn(OH)2, aluminium oxide, zinc phosphate, or calcium phosphate, method evaluation, two fractions, overview
-
isolation of glyco-GOD and aglyco-GOD from tunicamycin containing growth medium
-
native enzyme 10.7fold by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
native enzyme 26.4fold by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
-
native enzyme 63fold by ammonium sulfate fractionation, ultrafiltration, anion exchange chromatography, and gel filtration, to 93% purity
-
native enzyme 9.5fold from strain PIL7 by ammonium sulfate fractionation, dialysis, anion exchange chromatography, and gel filtration
-
native enzyme by ammonium sulfate fractionation, ion exchange chromatography, and gel filtration
-
native enzyme by liquid-liquid cationic reversed micelles extraction, CTAB micelles, method evaluation, overview
-
native enzyme to homogeneity
-
native extracellular enzyme by step-by-step ultrafiltration
-
of the reactivated recombinant enzyme, using mild acidification and anion-exchange chromatography on Q-Sepharose
-
one step purification
partial
phenyl Sepharose column chromatography and Q Sepharose column chromatography
-
phenyl Sepharose column chromatography, Q Sepharose column chromatography, gel filtration
-
purchased and further purified by DEAE-Toyopearl 650M column chromatography
-
purification to homogeneity by hydrophobic interaction and ion-exchange chromatography
recombinant enzyme 2.4fold from Pichia pastoris culture supernatant by ultrafiltration, anion exchange chromatography, and gel filtration
recombinant extracellular enzyme 1.26fold from Pichia pastoris by anion exchange chromatography
recombinant mutant enzyme B11 from Saccharomyces cerevisiae strain EBY100 cell walls by anion exchange chromatography and ultrafiltration
recombinant protein, gel filtration
-
recombinant wild-type and mutant M12 from Pichia pastoris strain KM71H by cross-flow ultrafiltration and anion exchange chromatography
Sephacryl 200 gel filtration
-
Sephadex G-50 gel filtration
-
ultrafiltration, combined treatment with salts and Triton X-100 is followed by a significant increase in the effectiveness of ultrafiltration purification of the culture fluid
-
using ammonium sulfate precipitation, gel filtration, anion-exchange and hydrophobic-interaction chromatography
-
using an extraction step of the mycelium mass at pH 3.0, cation exchange chromatography on S-Sepharose and gel filtration on Sephacryl S-300
-
using column chromatography on DEAE-Sephadex, Sephacryl S-300 and DEAE-Sepharose
-
using dialysis, ammonium sulfate fractionation and column chromatography on DEAE-cellulose
-
using high performance hydrophobic-interaction chromatography on a pentylagarose column
-
using ion-exchange chromatography on a Mono Q HR 5/5 column
-
using ion-exchange chromatography on DEAE-Sepharose and gel filtration chromatography on Sephacryl S-300
-