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1.1.3.38: vanillyl-alcohol oxidase

This is an abbreviated version!
For detailed information about vanillyl-alcohol oxidase, go to the full flat file.

Word Map on EC 1.1.3.38

Reaction

Vanillyl alcohol
+
O2
=
Vanillin
+
H2O2

Synonyms

4-allylphenol oxidase, 4-hydroxy-2-methoxybenzyl alcohol oxidase, Aryl-alcohol oxidase, EUGO, HMFO, Oxidase, vanillyl alcohol, RHA1_ro03282, vanillyl-alcohol oxidase, VAO, VAOA

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.38 vanillyl-alcohol oxidase

Crystallization

Crystallization on EC 1.1.3.38 - vanillyl-alcohol oxidase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structure of the enzyme in the native state and in complexes with the four inhibitors p-cresol, isoeugenol, 2-nitro-p-cresol and heptenyl-phenol
-
crystals of mutant enzyme D170S are grown using hanging-drop vapor diffusion method
-
crystals of mutant enzyme D170S/T457E are grown by using the hanging-drop vapor diffusion method
hanging-drop vapor diffusion method, apoenzyme, ADP-complex and holoenzyme. Crystal structure of both the holo and apo form of H61T are highly similar to the structure of the wild-type enzyme
hanging-drop vapor diffusion method. Crystal structures of mutant enzymes I238T, F454Y, E502G and T505S are highly similar to that of wild-type enzyme
tyrosine residues Tyr-108 and Tyr-503 are positioned to facilitate deprotonation of substrate's phenol groups