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1.1.3.38: vanillyl-alcohol oxidase

This is an abbreviated version!
For detailed information about vanillyl-alcohol oxidase, go to the full flat file.

Word Map on EC 1.1.3.38

Reaction

Vanillyl alcohol
+
O2
=
Vanillin
+
H2O2

Synonyms

4-allylphenol oxidase, 4-hydroxy-2-methoxybenzyl alcohol oxidase, Aryl-alcohol oxidase, EUGO, HMFO, Oxidase, vanillyl alcohol, RHA1_ro03282, vanillyl-alcohol oxidase, VAO, VAOA

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.38 vanillyl-alcohol oxidase

Cloned

Cloned on EC 1.1.3.38 - vanillyl-alcohol oxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21 cells
expressed in the vanillin-tolerant Gram-positive Amycolatopsis spec. HR167
-
expression in Escherichia coli
mutant enzymes I238T, F454Y, E520G and T505S are overexpressed in Escherichia coli
overexpression of apoVAO in Escherichia coli strain BSV11, which is defective in riboflavin synthesis. Incubation of apoVAO with FAD results in full restoration of enzyme activity dependent on FAD concentration, displaying a hyperbolic relationship, overview