1.1.3.10: pyranose oxidase
This is an abbreviated version!
For detailed information about pyranose oxidase, go to the full flat file.
Word Map on EC 1.1.3.10
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1.1.3.10
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trametes
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multicolor
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1,4-benzoquinone
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chrysosporium
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phanerochaete
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white-rot
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nivale
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microdochium
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l-sorbose
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aldopyranoses
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synthesis
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1,5-anhydro-d-glucitol
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flavinylated
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ligninolytic
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ochracea
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glucose-methanol-choline
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1,5-anhydroglucitol
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peniophora
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c4a-hydroperoxyflavin
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biotechnology
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food industry
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energy production
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biofuel production
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analysis
- 1.1.3.10
- trametes
- multicolor
- 1,4-benzoquinone
- chrysosporium
- phanerochaete
-
white-rot
- nivale
-
microdochium
- l-sorbose
- aldopyranoses
- synthesis
- 1,5-anhydro-d-glucitol
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flavinylated
-
ligninolytic
- ochracea
-
glucose-methanol-choline
- 1,5-anhydroglucitol
- peniophora
-
c4a-hydroperoxyflavin
- biotechnology
- food industry
- energy production
- biofuel production
- analysis
Reaction
Synonyms
C-2 specific pyranose-2-oxidase, carbohydrate oxidase, glucose 2-oxidase, glucose-2-oxidase, P2O, P2Ox, POX, PROD, PyOx, pyranose 2-Oxidase, pyranose oxidase, pyranose-2-oxidase, pyranose/oxygen 2-oxidoreductase, pyranose: oxygen 2-oxidoreductase, pyranose:oxygen 2-oxidoreductase, pyranose:oxygen-2-oxidoreductase, TmP2Ox
ECTree
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Purification
Purification on EC 1.1.3.10 - pyranose oxidase
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ammonium sulfate precipitation, phenyl Sepharose 6 column chromatography, DEAE-Sepharose column chromatography, and Sephacryl S-300 gel filtration
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homogenized in a French press, one-step immobilized metal affinity chromatography, concentrated in 50mM potassium phosphate buffer
IMAC Ni-charged resin column chromatography
Ni-NTA agarose resin column chromatography and Superdex 200 gel filtration
Ni2+ immobilized column with Chelating Sepharose Fast Flow combined with Äkta Purifier System, concentrated in an Amicon Ultra Centrifugal Filter Device
partial purification, PEG 4000 precipitation, DEAE-Sepharose column chromatography, and gel filtration
Polyporus obtusus
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protein purification by immobilized metal affinity chromatography, ultrafiltration, concentration of enzymes
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recmbinant enzyme from Escherichia coli by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
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recombinant His-tagged enzyme 232fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
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recombinant His-tagged enzyme 6.4fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
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recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinit chromatography and ulrafiltration
recombinant protein, gel purification and SDS-PAGE
stored, wet cell paste is treated with lysozyme, PMSF, EDTA buffer. Following steps are RNAse and DNAse treatment, sonication, dialyse, DEAE Sepharose Fast Flow column, dialyse, Phenyl Sepharose Fast Flow column, concentration by ammonium sulfate saturation, final dialyse. Stored at 20°C
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using ammonium sulfate fractionation, hydrophobic interaction, ion-exchange chromatography, and gel filtration
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using ammonium sulfate treatment, column chromatography on phenyl-Sepharose CL-4B, DEAE-Sephacel and chromatofocusing
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using column chromatography on HPA-75, Phenyl-Sepharose CL-6B and cellulofine GCL-2000 superfine
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using column chromatography on HPA-75, Sepharose 4B, Sephadex G-100 gel filtration and hydroxylapatite chromatography
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using fractional precipitation with polyethylene glycol and acid treatment
Polyporus obtusus
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using fractionated ammonium sulfate precipitation, chromatography on DEAE-Sephacel, Sephacryl S 300, S Sepharose and Q Sepharose
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using heat treatment of the mycelium extract and immunoaffinity chromatography
Phanerochaete gigantea
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using heat treatment, immunoaffinity chromatography and gel filtration on Superdex 200
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using hydrophobic interaction chromatography on phenyl-Sepharose CL-4B, anion-exchange chromatography on a Mono-Q column and chromatofocusing on a Mono-P column
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using liquid chromatography on phenyl Sepharose, Mono Q twice and phenyl Superose
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