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BRENDA support

1.1.1.B3: (S)-specific secondary alcohol dehydrogenase

This is an abbreviated version!
For detailed information about (S)-specific secondary alcohol dehydrogenase, go to the full flat file.

Reaction

(S)-R-CHOH-R'
+
NAD+
=
R-CO-R'
+
NADH
+
H+

Synonyms

(S)-1-phenylethanol dehydrogenase, (S)-1-phenylethanolsynthase, acetophenone reductase, ADH, ADH-A, ADH1, ADH2, ADHTt, APRD, carbonyl reductase (NADH, specific for (S)-configuration of alcohol), CRII, More, NAD+-dependent (S)-stereospecific alcohol dehydrogenase, NAD+-dependent ADH, PED, S-ADH, SADH, Scr2, SDR, short chain dehydrogenase, short-chain NAD(H)-dependent alcohol dehydrogenase, SOU1, SPES, TtADH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.B3 (S)-specific secondary alcohol dehydrogenase

Purification

Purification on EC 1.1.1.B3 - (S)-specific secondary alcohol dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by heat treatment at 70°C and/or adsorption chromatography on an IDA-Cu2+ support, both methods combined result in 8.8fold purification
-
Ni Sepharose column chromatography
recombinant enzyme
recombinant enzyme 11fold to homogeneity from Escherichia coli strain BL21(DE3) by heat denaturation of host proteins, anion exchange chromatography and gel filtration
-
recombinant protein
-
Strep-Tactin column chromatography
-