1.1.1.B18: L-1-amino-2-propanol dehydrogenase

This is an abbreviated version!
For detailed information about L-1-amino-2-propanol dehydrogenase, go to the full flat file.

Reaction

Synonyms

AADH, amino alcohol dehydrogenase, L-1-amino-2-propanol dehydrogenase, NADP+-dependent aminoalcohol dehydrogenase

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.B18 L-1-amino-2-propanol dehydrogenase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
2	AA Sequence
4	Application
4	CAS Registry Number
3	Cloned(Commentary)
3	Cofactor
6	Expression
4	General Information
1	Inhibitors
5	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
18	Organism
2	pH Optimum
2	Protein Variants
1	Purification (Commentary)
1	Reaction
6	Reaction Type
9	Reference
1	Specific Activity [micromol/min/mg]
15	Substrates and Products (Substrate)
6	Subunits
11	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]

Cloned

print visible entries

print all entries

Cloned on EC 1.1.1.B18 - L-1-amino-2-propanol dehydrogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide Go to Cloned (commentary) Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

construction of Rhodococcus expression vectors, derived from the Rhodococcus/Escherichia coli shuttle vector pRET1102, to express aadh. The plasmid pRET1102 can be transformed into many actinomycete strains, including Rhodococcus erythropolis. The transformation efficiency for a species closely related to Rhodococcus erythropolis is higher than that for other actinomycete strains. Promoters of various strengths, hsp, 1200rep, and TRR, are obtained from Gram-positive bacteria. The activity of TRR is stronger than that of hsp and 1200rep. The aadh-expressing plasmid pRET1172 with TRR can be transformed into many actinomycete strains to increase their AADH production. The Rhodococcus expression vector, pRET11100, constructed by removing aadh from the pRET1172 plasmid may be useful for bioconversion

Rhodococcus erythropolis

A1IG83

762822

gene aadh, DNA and amino acid sequence determination and analysis, expression analysis

Rhodococcus erythropolis

A1IG83

724052

gene aadh, DNA and amino acid sequence determination and analysis, expression in Escherichia coli JM109 leading to a 10.4fold increase in specific activity as to catalysis of the reduction of (2S)-2-(methylamino)-1-phenylpropan-1-one, as compared with that of Rhodococcus erythropolis strain MAK154 induced by 1-amino-2-propanol. Coexpression of aadh with glucose dehydrogenase gene gdh as cofactor regeneration enzyme, and installation of a system for sufficient production of d-psi-pseudoephedrine from racemic (S)-1-phenyl-2-methylaminopropan-1-one

Rhodococcus erythropolis

A1IG83

695805