1.1.1.85: 3-isopropylmalate dehydrogenase This is an abbreviated version! For detailed information about 3-isopropylmalate dehydrogenase, go to the full flat file .
Reaction
(2R,3S)-3-isopropylmalate +
NAD+ =
(2S)-2-isopropyl-3-oxosuccinate +
NADH +
H+
Synonyms 2-hydroxy-4-methyl-3-carboxyvalerate:NAD+ oxidoreductase, 2R,3S-isopropylmalate:NAD+ oxidoreductase (decaboxylating), 3-IPM dehydrogenase, 3-IPM-DH, 3-isopropylmalate dehydrogenase, 3-isopropylmalateDH, APS IPMDH, beta-IPM dehydrogenase, beta-IPMDH, beta-isopropylmalate dehydrogenase, beta-isopropylmalic enzyme, Bs3-isopropylmalateDH, dehydrogenase, 3-isopropylmalate, IMDH, IPMDH, IPMDH1, IPMDH2, IPMDH3, isopropylmalate dehydrogenase, leuB, NAD-dependent isopropylmalate dehydrogenase, Saci_0600, SbIPMDH, SoIPMDH, threo-Ds-3-isopropylmalate dehydrogenase, Tt-beta-3-isopropylmalateDH, two-domain 3-isopropylmalate dehydrogenase, Ydr417cp
ECTree
Temperature Range
Temperature Range on EC 1.1.1.85 - 3-isopropylmalate dehydrogenase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
20 - 40
-
the van t Hoff plot of Km IPM shows sigmoid-like transition within this range
25 - 50
-
although the ancestral IPMDHs (ancIPMDH-IQ and ancIPMDH-ML) are slightly less catalytically active relative to the mesophilic IPMDHs, they are more active than the thermophilic IPMDH at temperatures ranging from 25 to 50°C. ancIPMDH-IQ and ancIPMDH-ML functioned optimally at 70-80°C and maintain, respectively, 7% and 4% of their maximum activities at 25°C
25 - 95
2% of maximal activity at 25°C, maximal activity at 95°C
40 - 80
-
40°C: about 45% of maximal activity, 80°C: about 85% of maximal activity