1.1.1.82: malate dehydrogenase (NADP+)
This is an abbreviated version!
For detailed information about malate dehydrogenase (NADP+), go to the full flat file.
Word Map on EC 1.1.1.82
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1.1.1.82
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chloroplast
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nadp-malate
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dikinase
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3.1.3.11
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ferredoxin-thioredoxin
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agriculture
- 1.1.1.82
- chloroplast
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nadp-malate
- dikinase
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3.1.3.11
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ferredoxin-thioredoxin
- agriculture
Reaction
Synonyms
(S)-malate dehydrogenase, dehydrogenase, malate (nicotinamide adenine dinucleotide phosphate), L-malate:NAD oxidoreductase, malate NADP dehydrogenase, malic dehydrogenase (nicotinamide adenine dinucleotide phosphate), MDH, NADP malate dehydrogenase, NADP+-dependent malate dehydrogenase, NADP-dependent malate dehydrogenase, NADP-linked malate dehydrogenase, NADP-malate dehydrogenase, NADP-malic enzyme, NADP-MDH, NADP-MDH1, NADP-MDH2, NADPH-MDH
ECTree
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Renatured
Renatured on EC 1.1.1.82 - malate dehydrogenase (NADP+)
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renaturation of the guanidine-HCl denatured enzyme is more rapid with the reduced enzyme and occurs with higher yields, 100%, than with the oxidized enzyme, 60-80%
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solubilization and refolding of recombinant MDH from Escherichia coli inclusion bodies, the enzyme is dissolved in 6 M guanidine-HCl and gradually refolded to the active enzyme through dilution of the denaturant, conditions: refolding buffer 1.5 L of 0.1 M Tris-HCl, pH 7.5, containing 2 mM EDTA and 0.4 M L-arginine, incubation for 36 h at 4°C