1.1.1.81: hydroxypyruvate reductase

This is an abbreviated version!
For detailed information about hydroxypyruvate reductase, go to the full flat file.

Reaction

Synonyms

At1g12550, At1g79870, AtHPR1, AXX17_At1g12940, beta-hydroxypyruvate reductase, cytosolic hydroxypyruvate reductase, D-2-hydroxy-acid dehydrogenase, D-glycerate dehydrogenase, DVE02_v2_12566, GhrA, GhrB, glycerate dehydrogenase, glyoxylate reductase/hydroxypyruvate reductase, glyoxylate/hydroxypyruvate reductase, glyoxylate/hydroxypyruvate reductase A, GLYR1, GRHPR, HPPR2, HPPR3, HPR, HPR1, HPR2, HPR3, HRP, hydroxypyruvate reductase, LCv4_3219, MCA1407, More, NAD(P)H-dependent hydroxypyruvate reductase, NADH-dependent hydroxypyruvate reducing enzyme activity, NADH-dependent hydroxypyruvate reductase, NADH-HPR, NADH:hydroxypyruvate reductase, NADPH-dependent hydroxypyruvate reductase, SMc02828, SMc04462

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.81 hydroxypyruvate reductase

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Results	in table
41	Activating Compound
6364	AA Sequence
3	Application
1	CAS Registry Number
19	Cloned(Commentary)
47	Cofactor
7	Crystallization (Commentary)
5	Disease/ Diagnostics
1	Expression
28	General Information
2	General Stability
73	Inhibitors
45	kcat/KM [mM/s]
4	Ki Value [mM]
82	KM Value [mM]
16	Localization
11	Molecular Weight [Da]
62	Natural Substrates/ Products (Substrates)
78	Organism
8	Pathway
36	PDB ID
14	pH Optimum
2	pH Range
18	Protein Variants
9	Purification (Commentary)
1	Reaction
3	Reaction Type
59	Reference
48	Source Tissue
24	Specific Activity [micromol/min/mg]
2	Storage Stability
140	Substrates and Products (Substrate)
11	Subunits
82	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
1	Temperature Stability [°C]
39	Turnover Number [1/s]

KM Value

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KM Value on EC 1.1.1.81 - hydroxypyruvate reductase

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

11

2-dehydro-D-gluconate

Sinorhizobium meliloti

Q92LZ4, Q92T34

pH 7.5, 25°C

760598

0.057 - 9.35

3-hydroxypyruvate

8 entries

0.8

4-hydroxyphenylpyruvate

Arabidopsis thaliana

A0A178WMD4, Q9CA90

pH 7.0, 30°C, recombinant enzyme

761180

0.522

D-glycerate

Bos taurus

-

D-glycerate oxidation, 50 mM, NaCl, pH 9

287383

1.4

DL-glycerate

Bos taurus

-

-

287379

2.6

glycerate

Methylorubrum extorquens AM1

-

-

286508

3 - 80

glyoxylate

7 entries

0.0038 - 40

Hydroxypyruvate

27 entries

0.077 - 0.22

NAD+

2 entries

0.0061 - 0.24

NADH

14 entries

0.037

NADP+

Bos taurus

-

-

287379

0.0105 - 0.22

NADPH

12 entries

1.5 - 6.58

phenylpyruvate

5 entries

9.69

pyruvate

Sinorhizobium meliloti

Q92LZ4, Q92T34

pH 7.5, 25°C

760598

0.057

3-hydroxypyruvate

Methylococcus capsulatus

Q608T2

cosubstrate NADH, pH 7, 37°C

760546

0.14

3-hydroxypyruvate

Methylosinus trichosporium

-

cosubstrate NADH, pH 7, 30°C

760546

0.17

3-hydroxypyruvate

Methylotuvimicrobium alcaliphilum

-

cosubstrate NADPH, pH 7, 37°C

760546

0.5

3-hydroxypyruvate

Methylococcus capsulatus

Q608T2

cosubstrate NADPH, pH 7, 37°C

760546

0.6

3-hydroxypyruvate

Methylotuvimicrobium alcaliphilum

-

cosubstrate NADH, pH 7, 37°C

760546

1.2

3-hydroxypyruvate

Methylosinus trichosporium

-

cosubstrate NADPH, pH 7, 30°C

760546

6.14

3-hydroxypyruvate

Sinorhizobium meliloti

Q92LZ4, Q92T34

K0.5 value, Hill coefficient 1.74, pH 7.5, 25°C

760598

9.35

3-hydroxypyruvate

Sinorhizobium meliloti

Q92LZ4, Q92T34

pH 7.5, 25°C

760598

3

glyoxylate

Methylotuvimicrobium alcaliphilum

-

cosubstrate NADH, pH 7, 37°C

760546

3.9

glyoxylate

Methylococcus capsulatus

Q608T2

cosubstrate NADH, pH 7, 37°C

760546

11

glyoxylate

Methylotuvimicrobium alcaliphilum

-

cosubstrate NADPH, pH 7, 37°C

760546

12.3

glyoxylate

Sinorhizobium meliloti

Q92LZ4, Q92T34

K0.5 value, Hill coefficient 1.83, pH 7.5, 25°C

760598

18

glyoxylate

Methylosinus trichosporium

-

cosubstrate NADPH, pH 7, 30°C

760546

22

glyoxylate

Methylosinus trichosporium

-

cosubstrate NADH, pH 7, 30°C

760546

80

glyoxylate

Sinorhizobium meliloti

Q92LZ4, Q92T34

K0.5 value, Hill coefficient 1.48, pH 7.5, 25°C

760598

0.0038

Hydroxypyruvate

Bos taurus

-

value increases with NaCl concentrations higher than 10 mM

287383

0.004

Hydroxypyruvate

Bos taurus

-

-

287379

0.02

Hydroxypyruvate

Pyrococcus horikoshii

-

with NADPH as cosubstrate, at pH 8.0 and 50°C

739674

0.07

Hydroxypyruvate

Pyrococcus horikoshii

-

with NADH as cosubstrate, at pH 8.0 and 50°C

739674

0.08

Hydroxypyruvate

Hordeum vulgare

-

enzyme activity of wild-type in 40-60% precipitate fractions after ammonium sulfate fractionation

287368

0.09

Hydroxypyruvate

Pyrococcus furiosus

Q8U3Y2

with NADPH as cosubstrate, at pH 8.0 and 50°C

739674

0.1

Hydroxypyruvate

Bos taurus

-

-

287376

0.1

Hydroxypyruvate

Methylorubrum extorquens AM1

-

cofactor NADH

286508

0.11

Hydroxypyruvate

Zea mays

-

ammonium sulfate fractionated leaf extracts, 45-60% fraction

287370

0.13

Hydroxypyruvate

Delftia acidovorans

-

-

287382

0.13

Hydroxypyruvate

Delftia acidovorans

-

with 0.15 mM NADPH

287381

0.14

Hydroxypyruvate

Pyrococcus furiosus

Q8U3Y2

with NADH as cosubstrate, at pH 8.0 and 50°C

739674

0.165

Hydroxypyruvate

Pyrococcus yayanosii

F8AEA4

with NADPH as cosubstrate, at pH 8.0 and 50°C

739674

0.175

Hydroxypyruvate

Hyphomicrobium methylovorum GM2

-

native enzyme

287364

0.189

Hydroxypyruvate

Hyphomicrobium methylovorum GM2

-

purified recombinant enzyme

287364

0.41

Hydroxypyruvate

Pyrococcus yayanosii

F8AEA4

with NADH as cosubstrate, at pH 8.0 and 50°C

739674

0.71

Hydroxypyruvate

Hordeum vulgare

-

enzyme activity of LaPr 88/29 mutant which lacks NADH-prefering hydroxypyruvate reductase in 40-60% precipitate fractions after ammonium sulfate fractionation

287368

0.8

Hydroxypyruvate

Spinacia oleracea

-

at pH 6.0

287374

1.05

Hydroxypyruvate

Arabidopsis thaliana

A0A178WMD4, Q9CA90

pH 7, 30°C

761180

1.05

Hydroxypyruvate

Arabidopsis thaliana

A0A178WMD4, Q9CA90

pH 7.0, 30°C, recombinant enzyme

761180

1.25

Hydroxypyruvate

Methylorubrum extorquens AM1

-

cofactor NADPH

286508

2.1

Hydroxypyruvate

Delftia acidovorans

-

-

287382

2.1

Hydroxypyruvate

Delftia acidovorans

-

with 0.15 mM NADH

287381

3.57

Hydroxypyruvate

Arabidopsis thaliana

A0A178WMD4, Q9CA90

pH 7, 30°C

761180

6.1

Hydroxypyruvate

Arabidopsis thaliana

A0A178WMD4, A0A1I9LPQ6, Q9C9W5, Q9CA90, Q9LE33

GLYR1, at pH 7.8 and 22°C

737745

10

Hydroxypyruvate

Methylorubrum extorquens AM1

-

enzyme in high-salt fraction of DEAE-cellulose chromatography

287369

40

Hydroxypyruvate

Methylorubrum extorquens AM1

-

enzyme in low-salt fraction of DEAE-cellulose chromatography

287369

0.077

NAD+

Bos taurus

-

-

287379

0.22

NAD+

Bos taurus

-

D-glycerate oxidation, 50 mM, NaCl, pH 9

287383

0.0061

NADH

Bos taurus

-

-

287383

0.013

NADH

Pyrococcus horikoshii OT3

-

with hydroxypyruvate as substrate, pH 7.5

684141

0.013

NADH

Methylosinus trichosporium

-

cosubsubstrate glyoxylate, 30°C, pH 7

760546

0.015

NADH

Pyrococcus horikoshii OT3

-

with glyoxylate as substrate, pH 7.5

684141

0.015

NADH

Methylococcus capsulatus

Q608T2

cosubsubstrate glyoxylate, 37°C, pH 7

760546

0.028

NADH

Methylosinus trichosporium

-

cosubsubstrate 3-hydroxypyruvate, 30°C, pH 7

760546

0.03

NADH

Methylococcus capsulatus

Q608T2

cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7

760546

0.04

NADH

Methylorubrum extorquens AM1

-

-

286508

0.055

NADH

Hyphomicrobium methylovorum GM2

-

native enzyme

287364

0.064

NADH

Hyphomicrobium methylovorum GM2

-

purified recombinant enzyme

287364

0.07

NADH

Methylotuvimicrobium alcaliphilum

-

cosubsubstrate glyoxylate, 37°C, pH 7

760546

0.08

NADH

Methylotuvimicrobium alcaliphilum

-

cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7

760546

0.112

NADH

Bos taurus

-

-

287376

0.24

NADH

Delftia acidovorans

-

-

287381

0.0105

NADPH

Sinorhizobium meliloti

Q92LZ4, Q92T34

pH 7.5, 25°C

760598

0.015

NADPH

Delftia acidovorans

-

-

287381

0.018

NADPH

Bos taurus

-

-

287376

0.021

NADPH

Pyrococcus horikoshii OT3

-

with glyoxylate as substrate, pH 7.5

684141

0.025

NADPH

Pyrococcus horikoshii OT3

-

with hydroxypyruvate as substrate, pH 7.5

684141

0.028

NADPH

Methylotuvimicrobium alcaliphilum

-

cosubsubstrate glyoxylate, 37°C, pH 7

760546

0.055

NADPH

Methylotuvimicrobium alcaliphilum

-

cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7

760546

0.059

NADPH

Sinorhizobium meliloti

Q92LZ4, Q92T34

pH 7.5, 25°C

760598

0.06

NADPH

Methylorubrum extorquens AM1

-

-

286508

0.11

NADPH

Methylosinus trichosporium

-

cosubsubstrate 3-hydroxypyruvate, 30°C, pH 7

760546

0.16

NADPH

Methylococcus capsulatus

Q608T2

cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7

760546

0.22

NADPH

Methylosinus trichosporium

-

cosubsubstrate glyoxylate, 30°C, pH 7

760546

1.5 - 2

phenylpyruvate

Arabidopsis thaliana

A0A178WMD4, Q9CA90

pH 7, 30°C

761180

3.7

phenylpyruvate

Arabidopsis thaliana

A0A178WMD4, Q9CA90

pH 7, 30°C

761180

3.7

phenylpyruvate

Arabidopsis thaliana

A0A178WMD4, Q9CA90

pH 7.0, 30°C, recombinant enzyme

761180

5.59

phenylpyruvate

Sinorhizobium meliloti

Q92LZ4, Q92T34

pH 7.5, 25°C

760598

6.58

phenylpyruvate

Sinorhizobium meliloti

Q92LZ4, Q92T34

pH 7.5, 25°C

760598