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1.1.1.391: 3beta-hydroxycholanate 3-dehydrogenase (NAD+)

This is an abbreviated version!
For detailed information about 3beta-hydroxycholanate 3-dehydrogenase (NAD+), go to the full flat file.

Reaction

isolithocholate
+
NAD+
=
3-oxo-5beta-cholan-24-oate
+
NADH
+
H+

Synonyms

3beta-HSD, 3beta-HSDH, 3beta-hydroxysteroid dehydrogenase, bile acid 3beta-HSD, iso bile acid dehydrogenase, NAD-dependent 3beta-hydroxysteroid dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.391 3beta-hydroxycholanate 3-dehydrogenase (NAD+)

Specific Activity

Specific Activity on EC 1.1.1.391 - 3beta-hydroxycholanate 3-dehydrogenase (NAD+)

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.35
-
pH 9.5, 25°C, substrate 3,7-dioxo-5beta-cholan-24-oic acid
0.38
-
pH 9.5, 25°C, substrate 7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid
0.59
-
pH 9.5, 25°C, substrate 3,12-dioxo-5beta-cholan-24-oic acid
1
-
pH 9.5, 25°C, substrate 7alpha,12alpha-3-oxo-dihydroxy-5beta-cholan-24-oic acid
2.12
-
pH 9.5, 25°C, substrate 3,7,12-trioxo-5beta-cholan-24-oic acid
2.35
-
pH 9.5, 25°C, substrate 3,7-dioxo-5beta-cholan-24-oic acid
2.45
-
pH 9.5, 25°C, substrate 3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid
2.49
-
pH 9.5, 25°C, substrate 12alpha-hydroxy-3-oxo-5beta-cholate
3.2
-
purified enzyme, pH 10.0-10.2, 37°C