1.1.1.383: ketol-acid reductoisomerase [NAD(P)+]
This is an abbreviated version!
For detailed information about ketol-acid reductoisomerase [NAD(P)+], go to the full flat file.
Reaction
Synonyms
ilvC, Shewana3_0355
ECTree
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KCat KM Value
KCat KM Value on EC 1.1.1.383 - ketol-acid reductoisomerase [NAD(P)+]
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0.3
wild-type, pH not specified in the publication, temperature not specified in the publication
0.3
NADH
wild-type, pH 7.0, temperature not specified in the publication
0.43
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
2.5
NADH
-
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
2.6
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
2.7
NADH
mutant V48L/R49P/K52E/S53D, pH not specified in the publication, temperature not specified in the publication
3.2
NADH
-
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
3.7
NADH
mutant V48L/R49P/K52L/S53D, pH not specified in the publication, temperature not specified in the publication
9
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
9
NADH
mutant S61D/S63D, pH not specified in the publication, temperature not specified in the publication
11
NADH
mutant Q110A, pH 7.0, temperature not specified in the publication
14
NADH
mutant R76D/Ser78D, pH not specified in the publication, temperature not specified in the publication
22
NADH
mutant S61D/S63D/I95V, pH not specified in the publication, temperature not specified in the publication
24.8
NADH
mutant Q110V, pH 7.0, temperature not specified in the publication
27.6
NADH
mutant S78D, pH 7.0, temperature not specified in the publication
31.8
NADH
mutant S78D/Q110V, pH 7.0, temperature not specified in the publication
32
NADH
mutant A71S/R76D/S78AD/Q110V, pH not specified in the publication, temperature not specified in the publication
60.3
NADH
mutant A71S/R76D/S78D/Q110A, pH 7.0, temperature not specified in the publication
71
NADH
mutant V48L/R49P/K52L/S53D/E59K/T182S/E320K, pH not specified in the publication, temperature not specified in the publication
74
NADH
mutant A71S/R76D/S78D/Q110V, pH 7.0, temperature not specified in the publication
74
NADH
mutant A71S/R76D/S78D/Q110V, pH not specified in the publication, temperature not specified in the publication
165
NADH
mutant A71S/R76D/S78D/Q110V/D146G/G185R/K433E , pH not specified in the publication, temperature not specified in the publication
0.05
mutant V48L/R49P/K52E/S53D, pH not specified in the publication, temperature not specified in the publication
0.08
NADPH
mutant V48L/R49P/K52L/S53D, pH not specified in the publication, temperature not specified in the publication
0.11
NADPH
mutant S61D/S63D, pH not specified in the publication, temperature not specified in the publication
0.17
NADPH
mutant R76D/Ser78D, pH not specified in the publication, temperature not specified in the publication
0.25
NADPH
mutant S61D/S63D/I95V, pH not specified in the publication, temperature not specified in the publication
0.4
NADPH
mutant A71S/R76D/S78D/Q110V, pH not specified in the publication, temperature not specified in the publication
0.4
NADPH
mutant A71S/R76D/S78D/Q110V/D146G/G185R/K433E , pH not specified in the publication, temperature not specified in the publication
0.47
NADPH
mutant V48L/R49P/K52L/S53D/E59K/T182S/E320K, pH not specified in the publication, temperature not specified in the publication
0.5
NADPH
mutant A71S/R76D/S78AD/Q110V, pH not specified in the publication, temperature not specified in the publication
0.5
NADPH
mutant A71S/R76D/S78D/Q110V, pH 7.0, temperature not specified in the publication
1.1
NADPH
mutant A71S/R76D/S78D/Q110A, pH 7.0, temperature not specified in the publication
2.1
NADPH
-
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
2.7
NADPH
-
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
3.8
NADPH
mutant S78D, pH 7.0, temperature not specified in the publication
34.2
NADPH
mutant S78D/Q110V, pH 7.0, temperature not specified in the publication
65
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
87.3
NADPH
wild-type, pH 7.0, temperature not specified in the publication
88
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
302
NADPH
mutant Q110A, pH 7.0, temperature not specified in the publication
569
NADPH
mutant Q110V, pH 7.0, temperature not specified in the publication
800
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
4500
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
additional information
-
kcat/Km-value for NADH is above 20/mM*s, pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
additional information
NADPH
-
kcat/Km-value for NADPH is above 25/mM*s, pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate