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1.1.1.383: ketol-acid reductoisomerase [NAD(P)+]

This is an abbreviated version!
For detailed information about ketol-acid reductoisomerase [NAD(P)+], go to the full flat file.

Reaction

(2R)-2,3-dihydroxy-3-methylbutanoate
+
NAD(P)+
=
(2S)-2-hydroxy-2-methyl-3-oxobutanoate
+
NAD(P)H
+
H+

Synonyms

ilvC, Shewana3_0355

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.383 ketol-acid reductoisomerase [NAD(P)+]

Engineering

Engineering on EC 1.1.1.383 - ketol-acid reductoisomerase [NAD(P)+]

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A71S
mutant engineered to use NADH instead of NADPH
A71S/R76D/S78D/Q110A
mutant engineered to use NADH instead of NADPH
A71S/R76D/S78D/Q110V
A71S/R76D/S78D/Q110V/D146G/G185R/K433E
mutant engineered for reversal in cofactor specificity for NADH over NADPH
Q110A
mutant engineered to use NADH instead of NADPH
Q110V
mutant engineered to use NADH instead of NADPH
R76D
mutant engineered to use NADH instead of NADPH
S78D
mutant engineered to use NADH instead of NADPH
S78D/Q110V
mutant engineered to use NADH instead of NADPH
V48L/R49P/K52E/S53D
mutant engineered for reversal in cofactor specificity for NADH over NADPH
V48L/R49P/K52L/S53D
mutant engineered for reversal in cofactor specificity for NADH over NADPH
V48L/R49P/K52L/S53D/E59K/T182S/E320K
mutant engineered for reversal in cofactor specificity for NADH over NADPH
V48L/R49P/K52E/S53D
-
mutant engineered for reversal in cofactor specificity for NADH over NADPH
-
V48L/R49P/K52L/S53D
-
mutant engineered for reversal in cofactor specificity for NADH over NADPH
-
V48L/R49P/K52L/S53D/E59K/T182S/E320K
-
mutant engineered for reversal in cofactor specificity for NADH over NADPH
-
A71S/R76D/S78AD/Q110V
mutant engineered for reversal in cofactor specificity for NADH over NADPH
R76D/Ser78D
mutant engineered for reversal in cofactor specificity for NADH over NADPH
A71S/R76D/S78AD/Q110V
-
mutant engineered for reversal in cofactor specificity for NADH over NADPH
-
R76D/Ser78D
-
mutant engineered for reversal in cofactor specificity for NADH over NADPH
-
S61D/S63D
mutant engineered for reversal in cofactor specificity for NADH over NADPH
S61D/S63D/I95V
mutant engineered for reversal in cofactor specificity for NADH over NADPH
S61D/S63D
-
mutant engineered for reversal in cofactor specificity for NADH over NADPH
-
S61D/S63D/I95V
-
mutant engineered for reversal in cofactor specificity for NADH over NADPH
-
additional information
recipe for altering the cofactor specificity of the ketol-acid reductoisomerases (KARIs). Combining results for an engineered NADH-dependent variant of Escherichia coli KARI with available KARI crystal structures and a comprehensive KARI-sequence alignment, key cofactor specificity determinants are identified and used to construct KARIs with reversed cofactor preference