1.1.1.313: sulfoacetaldehyde reductase (NADPH)

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For detailed information about sulfoacetaldehyde reductase (NADPH), go to the full flat file.

Reaction

Synonyms

isethionate formation, reductase D, IsfD, IsfD2, NADPH-dependent sulfoacetaldehyde reductase

ECTree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.1.1 With NAD⁺ or NADP⁺ as acceptor

1.1.1.313 sulfoacetaldehyde reductase (NADPH)

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Results	in table
344	AA Sequence
2	Cloned(Commentary)
8	Cofactor
1	Crystallization (Commentary)
26	General Information
2	Inhibitors
2	kcat/KM [mM/s]
5	KM Value [mM]
3	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
12	Organism
3	Pathway
12	PDB ID
2	pH Optimum
1	pH Range
1	pH Stability
12	Protein Variants
2	Purification (Commentary)
1	Reaction
10	Reference
4	Source Tissue
16	Substrates and Products (Substrate)
3	Subunits
24	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Stability [°C]
2	Turnover Number [1/s]

Purification

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Purification on EC 1.1.1.313 - sulfoacetaldehyde reductase (NADPH)

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Mono Q column chromatography, phenyl Superose gel filtration

Chromohalobacter salexigens

709846

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by streptomycin sulfate precipitation, cobalt affinity chromatography, His-tag cleavage by TEV protease, dialysis, anion exchange chromatography, ultrafiltration, and gel filtration

Klebsiella oxytoca

D3U1D9

762706