1.1.1.31: 3-hydroxyisobutyrate dehydrogenase
This is an abbreviated version!
For detailed information about 3-hydroxyisobutyrate dehydrogenase, go to the full flat file.
Reaction
Synonyms
3-HIBADH, 3-hydroxyisobutyrate dehydrogenase, 3-hydroxyisobutyrate dehydrogenase-I, 3-hydroxyisobutyric acid dehydrogenase, 3HIBDH-IV, 3HIBDHI, At4g20930, AtHDH1, beta-hydroxyisobutyrate dehydrogenase, HDH1, HIBADH, HIBDH
ECTree
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Purification
Purification on EC 1.1.1.31 - 3-hydroxyisobutyrate dehydrogenase
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96fold purified at 4°C by ammonium sulfate fractionation and gel filtration
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ammonium sulfate precipitation, hydrophobic interaction chromatography (Phenyl-Sepharose), anion exchange chromatography
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native enzyme 100fold by ammonium sulfate fractionation, and hydrophobic interaction and anion exchange chromatography
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Ni-NTA column chromatography and Superdex 200 gel filtration
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Ni-NTA column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration
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Ni-NTA resin column chromatography