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1.1.1.289: sorbose reductase

This is an abbreviated version!
For detailed information about sorbose reductase, go to the full flat file.

Word Map on EC 1.1.1.289

Reaction

D-glucitol
+
NADP+
=
L-sorbose
+
NADPH
+
H+

Synonyms

FAD-dependent D-sorbitol dehydrogenase, flavin adenine dinucleotide-dependent D-sorbitol dehydrogenase, L-sorbose reductase, NADPH-dependent L-sorbose reductase, NADPH-dependent sorbose reductase, NADPH-SR, SboA, SLDH, SOU1

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.289 sorbose reductase

Cloned

Cloned on EC 1.1.1.289 - sorbose reductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Rosetta (DE3) cells
expression in Escherichia coli strain Rosseta (DE3)
-
genes sldSLC and sboA, DNA and amino acid sequence determination and analysis, genetic organization, a putational transcription regulator encoded by gene sboR is located upstream, sboRA comprises an operon, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
into the HindIII/NotI site of pET-28a(+) plasmid, expression construct is designed to overexpress only L-sorbose reductase protein (residues 1-263), overexpressed in Escherichia coli BL21 (DE3) cells