1.1.1.286: isocitrate-homoisocitrate dehydrogenase
This is an abbreviated version!
For detailed information about isocitrate-homoisocitrate dehydrogenase, go to the full flat file.
Reaction
Synonyms
3-carboxy-2-hydroxyadipate dehydrogenase, HICDH, homoisocitrate dehydrogenase, isocitrate-homoisocitrate dehydrogenase, More, Saci_2375, TK0280
ECTree
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KM Value
KM Value on EC 1.1.1.286 - isocitrate-homoisocitrate dehydrogenase
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0.05
pH 8.0, 60°C, recombinant His6-tagged mutant S80A
0.31
3-isopropylmalate
pH 8.0, 60°C, recombinant His6-tagged wild-type enzyme
0.68
3-isopropylmalate
pH 8.0, 60°C, recombinant His6-tagged mutant L81P
0.71
3-isopropylmalate
pH 8.0, 60°C, recombinant His6-tagged mutant T71V
0.0073
pH 8.0, 60°C, recombinant His6-tagged wild-type enzyme
0.037
homoisocitrate
pH 8.0, 60°C, recombinant His6-tagged mutant S80A
0.042
homoisocitrate
pH 8.0, 60°C, recombinant His6-tagged mutant L81P
0.17
homoisocitrate
pH 8.0, 60°C, recombinant His6-tagged mutant L83R
0.46
homoisocitrate
pH 8.0, 60°C, recombinant His6-tagged mutant I82N
0.94
homoisocitrate
pH 8.0, 60°C, recombinant His6-tagged mutant T71V
0.014
pH 8.0, 60°C, recombinant His6-tagged wild-type enzyme
0.3
isocitrate
pH 8.0, 60°C, recombinant His6-tagged mutant S80A
0.5
isocitrate
pH 8.0, 60°C, recombinant His6-tagged mutant T71V
0.58
isocitrate
pH 8.0, 60°C, recombinant His6-tagged mutant L83R
1.2
isocitrate
pH 8.0, 60°C, recombinant His6-tagged mutant L81P
2.2
isocitrate
pH 8.0, 60°C, recombinant His6-tagged mutant I82N
0.036
pH 8.0, 60°C, recombinant His6-tagged wild-type enzyme, with homoisocitrate
0.1
NAD+
pH 8.0, 60°C, recombinant His6-tagged wild-type enzyme, with isocitrate
0.16
NAD+
pH 8.0, 60°C, recombinant His6-tagged mutant L83R, with homoisocitrate
0.25
NAD+
pH 8.0, 60°C, recombinant His6-tagged mutant T71V, with isocitrate
0.32
NAD+
pH 8.0, 60°C, recombinant His6-tagged mutant L81P, with homoisocitrate
0.6
NAD+
pH 8.0, 60°C, recombinant His6-tagged mutant L81P, with isocitrate
0.62
NAD+
pH 8.0, 60°C, recombinant His6-tagged mutant L83R, with isocitrate
0.64
NAD+
pH 8.0, 60°C, recombinant His6-tagged mutant I82N, with homoisocitrate
0.76
NAD+
pH 8.0, 60°C, recombinant His6-tagged mutant S80A, with isocitrate
0.92
NAD+
pH 8.0, 60°C, recombinant His6-tagged mutant S80A, with homoisocitrate
1
NAD+
pH 8.0, 60°C, recombinant His6-tagged mutant T71V, with 3-isopropylmalate
1.2
NAD+
pH 8.0, 60°C, recombinant His6-tagged mutant S80A, with 3-isopropylmalate
1.3
NAD+
pH 8.0, 60°C, recombinant His6-tagged mutant L81P, with 3-isopropylmalate
1.5
NAD+
pH 8.0, 60°C, recombinant His6-tagged mutant T71V, with homoisocitrate
1.5
NAD+
pH 8.0, 60°C, recombinant His6-tagged wild-type enzyme, with 3-isopropylmalate
1.8
NAD+
pH 8.0, 60°C, recombinant His6-tagged mutant I82N, with isocitrate
additional information
Michaelis-Menten kinetics
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additional information
additional information
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kinetic mechanism, detailed overview
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